慢性应激对大鼠凝血功能和血小板活化的影响及电针的干预作用

doi:10.3969/j.issn.1006-9771.2017.03.011

Abstract

Abstract: ObjectiveTo observe the change of coagulation function in chronic stress depression model rats and the effects of electroacupuncture (EA) on it. MethodsA total of 32 Sprague-Dawley rats were randomly divided in control group (n=8), model group (n=8), EA group (n=8) and fluoxetine group (n=8) after a week of acclimation. The 21-day unpredictable mild stress combined with solitarily feeding was used to make the depression model in the latter three groups, meanwhile EA group and the fluoxetine group accepted EA and fluoxetine. They were measured the prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB). Serum levels of 6-keto-prostaglandin F_1α (6-keto-PGF_1α), P-selectin, thromboxane B₂ (TXB₂) were determined with enzyme-linked immunoabsorbent assay. ResultsThe cross and rear scores were significantly lower in the model group than in the control group (P<0.01). The scores increased in EA group and the fluoxetine group (P<0.01) compared with the model group. Compared with the control group, PT, APTT and TT decreased (P<0.01), FIB increased (P<0.05), the level of 6-keto-PGF_1α decreased, the level of P-selectin and TXB₂ increased (P<0.01) in the model group. Compared with the model group, PT, APTT and TT increased (P<0.05), the level of 6-keto-PGF_1α increased (P<0.01), and the level of P-selectin and TXB₂ decreased (P<0.05) in EA group and the fluoxetine group, and FIB decreased in EA group (P<0.05). ConclusionThe chronic stress depression rats are impaired in the coagulation function and platelet activation, and can be allayed with electroacupuncture and fluoxetine.

Key words: depression, electroacupuncture, coagulation function, platelet activation, rats

CLC Number:

R749.7

SONG Hong-tao, WU Zhong, ZHANG Rui-xia, SAIYIN Chao-ketu, JING Quan-kai, YAO Hai-jiang. Alteration of Coagulation Function and Platelet Activation in Chronicity Stress Depression Model Rats and Effect of Electroacupuncture[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(3): 304-307.

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Alteration of Coagulation Function and Platelet Activation in Chronicity Stress Depression Model Rats and Effect of Electroacupuncture

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