电针对APP/PS1小鼠学习记忆及β位淀粉样前体蛋白裂解酶1表达的影响

doi:10.3969/j.issn.1006-9771.2019.01.007

Abstract

Abstract: Objective To explore the effects of electroacupuncture at Baihui (DU20) and Shenting (GV24) on Alzheimer's disease, and possible mechanism for it.
Methods A total of 24 eight-month-old APP/PS1 male mice were randomly divided into model group (n = 8), electroacupuncture group (n = 8) and non-acupoint group (n = 8), and other eight wild-type mice were as wild-type group. The electroacupuncture group accepted electroacupuncture at Baihui and Shenting, while the non-acupoint group accepted electroacupuncture at bilateral subcostal non-acupoint area, and the wild-type group and the model group accepted the same grasping and fixing, for 28 days. Then they assessed with Morris water maze test. The levels of β-amyloid protein (Aβ) and β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in cerebral cortex were detected with immunohistochemistry and immunofluorescence respectively, and the level of BACE1 mRNA with RT-PCR.
Results Compared with the model group, the escape latency decreased in the electroacupuncture group (P < 0.001), and the times crossing platforms increased (P < 0.001), while the expression of BACE1 and Aβ decreased (P < 0.001).
Conclusion Electroacupuncture may improve the learning-memory ability by inhibiting the expression of BACE1 in the cerebral cortex of APP/PS1 mice to decreasing the level of Aβ.

Key words: Alzheimer's disease, electroacupuncture, learning memory, β-site amyloid precursor protein cleaving enzyme 1, β-amyloid, mice

CLC Number:

R749

HUANG Sheng, LI Long, WANG Zhi-fu, YANG Min-guang, LI Jian-hong, ZHANG Jia-yong, CHEN Li-dian. Effect of Electroacupuncture on Learning-memory and Expression of β-site Amyloid Precursor Protein Cleaving Enzyme 1 in APP/PS1 Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2019, 25(1): 44-50.

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Effect of Electroacupuncture on Learning-memory and Expression of β-site Amyloid Precursor Protein Cleaving Enzyme 1 in APP/PS1 Mice

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