《中国康复理论与实践》

中国康复理论与实践 >

2017 , Vol. 23 >Issue 3: 288 - 291

DOI: https://doi.org/10.3969/j.issn.1006-9771.2017.03.008

基础研究

运动或电针预处理对脑梗死大鼠神经功能缺损及层粘连蛋白表达的影响

  • 唐强 ,
  • 阮野 ,
  • 李宏玉 ,
  • 叶涛 ,
  • 吴孝军 ,
  • 田源 ,
  • 朱路文
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  • 1.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001;
    2.黑龙江中医药大学,黑龙江哈尔滨市 150040。
唐强(1963-),男,汉族,四川大竹县人,博士,教授,主要研究方向：神经系统疾病中医康复基础与临床研究。

收稿日期: 2016-11-15

  网络出版日期: 2017-03-31

基金资助

1.国家自然科学基金项目(No.81503666); 2.黑龙江中医药大学领军人才计划项目(No.2012RCL02); 3.黑龙江中医药大学研究生创新科研项目(No.yjscx2016037)

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Effects of Exercise or Electroacupuncture Preconditioning on Neurological Deficits and Expression of Laminin in Rats with Cerebral Infarction

  • TANG Qiang ,
  • RUAN Ye ,
  • LI Hong-yu ,
  • YE Tao ,
  • WU Xiao-jun ,
  • TIAN Yuan ,
  • ZHU Lu-wen
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  • 1. Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China;
    2. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China

Received date: 2016-11-15

  Online published: 2017-03-31

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摘要

目的探讨运动预处理与电针预处理对局灶性脑梗死大鼠神经功能的影响及其可能机制。方法24只雄性Sprague-Dawley大鼠随机分为模型组(n=6)、假手术组(n=6)、运动预处理组(n=6)和电针预处理组(n=6)。模型组、假手术组不行任何处理;运动预处理组行跑台训练2周,电针预处理组行电针预处理2周。采用改良线栓法制作大鼠局灶性脑梗死模型,假手术组行相同血管结扎,不造成梗死。24 h后采用改良神经功能缺损评分(NSS)对大鼠进行评定,Western blotting检测脑缺血区层粘连蛋白的表达。结果假手术组未见神经功能缺损表现,预处理组NSS评分低于模型组(P<0.05),两预处理组间无显著性差异(P>0.05)。假手术组层粘连蛋白表达最高,模型组较预处理组表达降低(P<0.05),两预处理组间无显著性差异(P>0.05)。结论运动预处理与电针预处理均能降低局灶性脑梗死大鼠神经功能缺损程度,可能与抑制层粘连蛋白早期表达降低有关。

关键词： 局灶性脑梗死; 预处理; 运动; 电针; 神经功能; 层粘连蛋白; 大鼠

本文引用格式

唐强 , 阮野 , 李宏玉 , 叶涛 , 吴孝军 , 田源 , 朱路文 . 运动或电针预处理对脑梗死大鼠神经功能缺损及层粘连蛋白表达的影响[J]. 中国康复理论与实践, 2017 , 23(3) : 288 -291 . DOI: 10.3969/j.issn.1006-9771.2017.03.008

Abstract

ObjectiveTo investigate the effect of electroacupuncture or exercise preconditioning on neurological function after focal cerebral infarction in rats and the possible mechanism. MethodsA total of 24 male Sprague-Dawley rats were randomly divided into model group (n=6), sham group (n=6), exercise preconditioning group (n=6) and electroacupuncture preconditioning group (n=6). The model group and the sham group did not accept any treatment, while the exercise preconditioning group and the electroacupuncture preconditioning group accepted treadmill training and electroacupuncture for two weeks, respectively. Their middle cerebral arteries were occluded with modified Longa's approach, except the sham group that was ligated the same arteries but did not result in infarction. They were evaluated with Neurologic Severity Scores (NSS) 24 hours after modeling, and the laminin expression in the ischemic area was detected with Western blotting. ResultsThere was no neurological deficit in the sham group. The NSS was lower in both preconditioning groups than in the model group (P<0.05), but was not significant different between preconditioning groups (P>0.05). The expression of laminin was the most in the sham group, and was more in both preconditioning groups than in the model group (P<0.05), but was not significant different between preconditioning groups (P>0.05). ConclusionPreconditioning with exercise or electroacupuncture can both reduce the neurological deficits in rats after focal cerebral infarction, which may associate with the protection of laminin from inhibition in early stage.

Key words： focal cerebral infarction; preconditioning; exercise; electroacupuncture; neurological function; laminin; rats

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