目的 探讨推拿手法联合跑台训练对坐骨神经横断伤延期外膜修复后促进神经再生的效果。方法 60只Sprague-Dawley大鼠随机等分为对照组、模型组和治疗组。后两组建立坐骨神经横断伤延期外膜缝合模型,治疗组给予捏法推拿及跑台训练,每天1次。分别于干预后1个月、2个月各取10只大鼠检测坐骨神经运动神经传导速度(MCV)、再生神经轴突数及施万细胞数,电镜观察超微结构。结果 与模型组比较,治疗组MCV在干预后2个月加快(P<0.05),轴突数无显著性差异(P>0.05),施万细胞数增加(P<0.05),电镜下损伤较轻。结论 推拿手法联合跑台训练可促进损伤的周围神经再生。
Objective To explore the effect of the combination of Tuina and treadmill training on nerve regeneration after sciatic nerve transection.Methods A total of 60 Sprague-Dawley rats were randomly divided into control group, model group and treatment group equally. The sciatic nerve was transected in the latter two groups and the epineurium suture was delayed four days. Then the treatment group accepted Tuina and treadmill training once a day. The motor conduction velocity (MCV) of sciatic nerve was measured one and two months after intervention in ten rats respectively, while the number of regenerated axons and Schwann cells was counted, and structures of sciatic never were observed under electron microscopy.Results Compared with the model group, the MCV accelerated in the treatment group (P<0.05), the number of axons was not significantly different (P>0.05), and the number of Schwann cells increased two months after modeling (P<0.05), with less injury under electron microscopy.Conclusion Tuina combined with treadmill training can promote the regeneration of the injured peripheral nerve.
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