目的 观察电针百会、大椎、肾俞穴对SAMP8小鼠学习记忆与海马CA1区神经元突触的影响,探讨电针治疗阿尔茨海默病(AD)的机制。方法 7月龄SAMP8小鼠24只随机分为模型组和电针组,同龄正常老化SAMR1小鼠12只为对照组。电针组电针百会、大椎、肾俞穴30 d。Morris水迷宫检测小鼠学习记忆能力,免疫组织化学观察小鼠海马CA1区突触素(SYN)及突触后致密区蛋白95 (PSD95)的表达,透射电镜观察小鼠海马CA1区突触超微结构的变化。结果 与模型组相比,电针组逃避潜伏期缩短(P<0.05),原平台象限停留时间延长(P<0.05),穿越原平台次数增加(P<0.05),海马CA1区SYN与PSD95表达显著提高(P<0.001),突触结构较完整,数量增加。结论 电针能提高小鼠海马CA1区神经元突触蛋白的表达,改善突触的超微结构,从而有效改善SAMP8小鼠的学习记忆能力。
Objective To observe the effect of electroacupuncture (EA) at Baihui (GV20), Dazhui (GV14) and Shenshu (BL23) acupoints on cognitive function and the synapse of neurons in hippocampal CA1 in SAMP8 mice, to explore the mechanism of EA in the treatment of Alzheimer's disease (AD).Methods A total of 24 seven-month-old SAMP8 mice were randomly divided into model group (n=12) and EA group (n=12), and the same age SAMR1 mice were as control group (n=12). The EA group accepted EA at Baihui, Dazhui and Shenshu for 30 days. They were assessed with Morris maze test. The expression of synaptophysin (SYN) and postsynaptic density protein 95 (PSD95) in hippocampal CA1 region were detected with immunohistochemistry. The morphology and density of synapse in hippocampal CA1 region was observed with transmission electron microscopy.Results Compared with the model group, the latency of Morris maze decreased in EA group (P<0.05), the time staying in the quadrant of the platform increased (P<0.05), as well as the number passing the original platform (P<0.05), with the more expression of SYN and PSD95 in hippocampal CA1 region (P<0.001), and more and completed synapse.Conclusion EA can improve the learning and memory ability of SAMP8 mice by increasing the expression of SYN and PSD95 to protect the ultrastructure of synapses in hippocampal CA1 region.
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