《中国康复理论与实践》

中国康复理论与实践 >

2019 , Vol. 25 >Issue 3: 249 - 254

DOI: https://doi.org/10.3969/j.issn.1006-9771.2019.03.001

专题

有氧运动对脑小血管病大鼠执行功能的效果

  • 王茹 ,
  • 刘楠 ,
  • 李卫萍 ,
  • 赵弘轶 ,
  • 杜菊梅 ,
  • 黄勇华
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  • 1.中国人民解放军总医院第七医学中心神经内科，北京市 100700
    2.陕西中医药大学第二附属医院，陕西咸阳市 712000
王茹(1991-)，女，汉族，陕西延安市人，硕士，主要研究方向：脑血管病。通讯作者：黄勇华(1969-)，男，广东梅州市人，博士，主要研究方向：脑血管疾病及睡眠障碍。

收稿日期: 2018-11-26

  修回日期: 2019-01-16

  网络出版日期: 2019-04-02

基金资助

国家自然科学基金青年项目(No. 81601035)

收起

Effect of Aerobic Exercise on Behavioral Function in Rats with Cerebral Small Vessel Disease

  • WANG Ru ,
  • LIU Nan ,
  • LI Wei-ping ,
  • ZHAO Hong-yi ,
  • DU Ju-mei ,
  • HUANG Yong-hua
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  • 1.Department of Neurology, the Seventh Medical Center of PLA General Hospital, Beijing 100700, China
    2.Department of Neurology, Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712000, China

Received date: 2018-11-26

  Revised date: 2019-01-16

  Online published: 2019-04-02

Supported by

National Natural Science Foundation of China (Youth) (No. 81601035)

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摘要

目的 探讨有氧运动改善脑小血管病大鼠认知执行能力的效果及作用机制。方法 8周龄雄性Sprague-Dawley大鼠随机分为假手术组(n = 16)、模型组(n = 16)和游泳组(n = 16)。双侧颈动脉结扎法造模。4周游泳锻炼后，各组行挖穴测试。Western blotting检测海马脑源性神经营养因子(BDNF)和原肌球蛋白受体激酶B (TrkB)蛋白水平。高尔基染色法观察海马神经元树突及突触棘的发育情况。免疫荧光法检测海马齿状回Ki67、双皮质素(DCX)和Neun的表达。结果 与模型组相比，游泳组挖穴能力更强(P < 0.05)，海马组织中BDNF和TrkB蛋白水平更高(P < 0.05)，海马齿状回Ki67/DCX和Neun阳性细胞表达率更高(P < 0.05)，海马区神经元树突范围更大，突触棘更长，神经元发育更好(P < 0.05)。结论 有氧运动通过BDNF/TrkB信号通路促进海马神经元的增殖分化，增加Ki67/DCX和Neun表达，促进海马神经元发育，改善脑小血管病大鼠执行功能。

关键词： 脑小血管病; 有氧运动; 海马; 执行功能; 脑源性神经营养因子; 原肌球蛋白受体激酶B; 大鼠

本文引用格式

王茹 , 刘楠 , 李卫萍 , 赵弘轶 , 杜菊梅 , 黄勇华 . 有氧运动对脑小血管病大鼠执行功能的效果[J]. 中国康复理论与实践, 2019 , 25(3) : 249 -254 . DOI: 10.3969/j.issn.1006-9771.2019.03.001

Abstract

Objective To explore the effect of aerobic exercise on behavioral function in rats with cerebral small vessel disease and its mechanism. Methods Eight-week-old male Sprague-Dawley rats were randomly divided into sham group (n = 16), model group (n = 16) and swimming group (n = 16). The model was developed with bilateral common carotid artery ligation. They were assessed with burrowing test after four weeks of swimming exercise. The levels of brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) protein in hippocampus were detected with Western blotting. The development of dendrites and synaptic spines in hippocampal neurons was observed with Golgi staining. The expression of Ki67, doublecortin (DCX) and Neun in hippocampal dentate gyrus was detected with immunofluorescence. Results Compared with the model group, the burrowing ability improved in the swimming group (P < 0.05), with increase of levels of BDNF and TrkB in hippocampus (P < 0.05), Ki67/DCX and Neun positive cells in hippocampal dentate gyrus (P < 0.05), and extension of dendrites and length of synaptic spine in hippocampal neurons (P < 0.05). Conclusion Aerobic exercise may promote the proliferation and differentiation of hippocampal neurons through BDNF/TrkB signaling pathway, expression of Ki67/DCX and Neun and development of hippocampal neurons, to improve behavioral function in rats with cerebral small vessel disease.

Key words： cerebral small vessel disease; aerobic exercise; hippocampus; behavioral function; brain-derived neurotrophic factor; tropomyosin receptor kinase B; rats

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