Objective To observe the effect of single kneeling position trunk intensive training on the balance of patients with stroke Pusher syndrome. Methods From January, 2014 to February, 2016, forty patients with stroke Pusher syndrome were randomly divided into observation group and control group equally. They all accepted routine rehabilitation, while the observation group added a trunk intensive training with one knee kneeled. They were assessed with Berg Balance Scale (BBS), Fugl-Meyer Assessment (FMA) of lower limb and Sheikh's Scale of Trunk Control before and after training. Results The scores of BBS, lower limb FMA and trunk control improved in both groups after eight weeks of treatment (t>1.756, P<0.05), and improved more in the observation group than in the control group (t>1.827, P< 0.05). Conclusion Single kneeling trunk intensive training can effectively improve the balance in stroke patients with Pusher syndrome.