Objective To explore the effect of particulate matter (PM) 2.5 on the expression of pigment epithelium- derived factor (PEDF) protein in bronchial epithelial cells (BEAS-2B). Methods Human BEAS-2B were subcultivated, followed by low, medium and high concentrations of PM2.5 (25 μg/ml, 50 μg/ml, 100 μg/ml) stimulation for 24 hours. The expression of PEDF protein in supernatant was analyzed by enzyme-linked immunosorbent assay (ELISA), and the expression in BEAS-2B cells was detected by Western blotting. Results Compared with the control group, the expression of PEDF protein in supernatant and BEAS-2B cells induced by PM2.5 (25 μg/ml) increased, but no significance was found (t=-0.730, t=-1.840, P>0.05), and the expression induced by PM2.5 with the concentrations of 50 μg/ml and 100 μg/ml significantly increased (t>5.798, P<0.05). Conclusion PM2.5 with the concentrations of 50 μg/ml and 100 μg/ml could increase the expression of PEDF protein in a concentration-dependent manner both in supernatant and BEAS-2B cells.