Objective To investigate the differential expression and gene functions of up-regulated genes in rats with spinal cord injury.Methods Female Sprague-Dawley rats' model of spinal cord injury was established with the modified Allen's method. Gene chip technology was used to detect the variation of differentially expressed genes in the spinal cord after spinal cord injury in rats. The differences in genes, functional localization and pathways were analyzed with gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway database.Results The results of total RNA quality in spinal cord segment were qualified. Gene chip results showed that there were 1874 differentially up-regulated genes and 2348 differentially down-regulated genes. Bioinformatics was used to analyze differentially up-regulated genes in terms of biological processes, cellular components, and molecular functions. The differentially up-regulated genes were involving apoptosis, immune response, inflammation, etc., pathway analysis mainly showed the differentially up-regulated genes involved phosphoinositide 3-kinase protein kinase B signaling pathway and Toll-like receptor signaling pathways.Conclusion Differentially up-regulated genes may be involved in secondary reactions following spinal cord injury, such as inflammation, immune response and hypoxia, and then further affect motor function and sensory function.
ZHONG Zhan-qiong
,
XUE Qing-cai
,
YI Xiao-hong
,
HUANG Cong
,
YU Hai-yan
,
LIU Wei-wei
,
CHEN Ji-lan
,
XIA Jun
,
ZHANG Xiao
. Bioinformatics Analysis of Up-regulated Differential Genes in Rats post Spinal Cord Injury[J]. Chinese Journal of Rehabilitation Theory and Practice, 2019
, 25(6)
: 621
-629
.
DOI: 10.3969/j.issn.1006-9771.2019.06.001
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