高频重复经颅磁刺激对大鼠工作记忆局部场电位和锋电位时频特征的影响

doi:10.3969/j.issn.1006-9771.2020.12.005

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (12): 1401-1409.doi: 10.3969/j.issn.1006-9771.2020.12.005

高频重复经颅磁刺激对大鼠工作记忆局部场电位和锋电位时频特征的影响

童禹铭,徐桂芝,郭苗苗(),张天恒,王宁

河北工业大学电气工程学院,a.省部共建电工装备可靠性与智能化国家重点实验室; b.天津市生物电工与智能健康重点实验室,天津市 300130

收稿日期:2019-10-23 修回日期:2019-12-23 出版日期:2020-12-25 发布日期:2020-12-30
通讯作者: 郭苗苗 E-mail:gmm@hebut.edu.cn
作者简介:童禹铭(1995-),男,汉族,湖北黄冈市人,硕士研究生,主要研究方向：生物电磁技术、脑认知与神经工程。
基金资助:
1.国家自然科学基金项目(51677053);1.国家自然科学基金项目(51737003);1.国家自然科学基金项目(51707054);2.河北省高等学校科学技术研究项目(QN2017048);3.河北省自然科学基金项目(F2017202197)

Effect of High Frequency Repetitive Transcranial Magnetic Stimulation on Local Field Potential and Spike Time-frequency Characteristics during Working Memory in Rats

TONG Yu-ming,XU Gui-zhi,GUO Miao-miao(),ZHANG Tian-heng,WANG Ning

a. State Key Laboratory of Reliability and Intelligence of Electrical Equipment; b. Tianjin Key Laboratory of Bioelectromagnetic Technology and Intelliaent Health, Tianjin 300130, China

Received:2019-10-23 Revised:2019-12-23 Published:2020-12-25 Online:2020-12-30
Contact: GUO Miao-miao E-mail:gmm@hebut.edu.cn
Supported by:
National Natural Science Foundation of China(51677053);National Natural Science Foundation of China(51737003);National Natural Science Foundation of China(51707054);Hebei Province Higher Education Science and Technology Research Project(QN2017048);Hebei Natural Science Foundation(F2017202197)

摘要/Abstract

摘要：

目的探究高频重复经颅磁刺激(rTMS)对大鼠学习记忆功能和神经电活动的影响。方法将8只Wistar大鼠随机分为对照组(n = 4)和刺激组(n = 4)。刺激组接受5 Hz rTMS,刺激强度为100%运动阈值,持续14 d,对照组未接受rTMS。在大鼠工作记忆实验过程中同步采集前额叶皮质(PFC)16通道的局部场电位信号(LFPs)和锋电位信号(spike)。对LFPs信号进行时频功率谱分析,对spike信号分析其发放速率,并利用Multitaper频谱相干方法分析LFPs-spike信号的同步化程度。结果工作记忆实验中,刺激组达到正确率标准所需时间少于对照组(t = 2.51, P = 0.046);刺激组LFPs的θ频段和γ频段能量均显著高于对照组(t> 12.49,P < 0.001),两组spike平均发放率无显著性差异( t = 1.73, P= 0.067),θ频段和γ频段LFPs-spike的同步化程度更高(t > 8.82, P < 0.001)。结论高频rTMS能够提升工作记忆实验中PFC脑区的LFPs信号能量,增强LFPs信号和spike信号的协同编码过程,并提高大鼠的工作记忆实验。

关键词: 重复经颅磁刺激, 高频, 工作记忆, 局部场电位, 锋电位, 大鼠

Abstract:

Objective To investigate the effects of high frequency repetitive transcranial magnetic stimulation (rTMS) on learning memory and neuroelectric activity in rats.Methods Eight Wistar rats were randomly divided into control group (n = 4) and rTMS group (n = 4). rTMS group was stimulated 14 days by rTMS with the frequency of 5 Hz, and the stimulation intensity was 100% motor threshold. The control group did not accepted rTMS. Then, the 16 channel local field potentials (LFPs) and spikes were recorded from their prefrontal cortex (PFC) in each group during the working memory tasks. The time-frequency analysis based on the short-time Fourier transform was performed on the LFPs, and the spike was analyzed by the release rate method. Multitaper Spectral Coherence was used to analyze the Synchronization Degree of LFPs-spike.Results In the working memory experiment, rTMS group needed less days than the control group to reach the task correction criterion (t = 2.51,P = 0.046). The energy intensity of the θ-band and γ-band of the LFPs was significantly higher in rTMS group than in the control group ( t> 12.49,P < 0.001), θ-band and γ-band LFPs-spike were more synchronized ( t > 8.82, P < 0.001), but there was no significant difference in the average rate of spike between groups ( t = 1.73, P= 0.067).Conclusion High frequency rTMS could increase the LFPs energy in working memory experiment, enhance cooperative coding process for LFPs and spike, and improve the working memory ability of the rats.

Key words: repetitive transcranial magnetic stimulation, high frequency, working memory, local field potentials, spike, rats

中图分类号:

R741

童禹铭,徐桂芝,郭苗苗,张天恒,王宁. 高频重复经颅磁刺激对大鼠工作记忆局部场电位和锋电位时频特征的影响[J]. 《中国康复理论与实践》, 2020, 26(12): 1401-1409.

TONG Yu-ming,XU Gui-zhi,GUO Miao-miao,ZHANG Tian-heng,WANG Ning. Effect of High Frequency Repetitive Transcranial Magnetic Stimulation on Local Field Potential and Spike Time-frequency Characteristics during Working Memory in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(12): 1401-1409.

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高频重复经颅磁刺激对大鼠工作记忆局部场电位和锋电位时频特征的影响

Effect of High Frequency Repetitive Transcranial Magnetic Stimulation on Local Field Potential and Spike Time-frequency Characteristics during Working Memory in Rats

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