《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (9): 834-836.

• 论文 • 上一篇    下一篇

地塞米松对内毒素休克大鼠脑组织细胞因子含量的影响

王新芳1，张宇忠2，任丽薇2，贾旭2，庞丹丹2   

  1. 1. 首都医科大学燕京医学院生理学与病理生理学教研室，北京市 101300；2. 北京中医药大学基础医学院病理教研室，北京市 100029。
  • 收稿日期:2011-04-28 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 张宇忠

Effect of Dexamethasone on Cytokine in Brain of Rats with Endotoxin Shock

WANG Xin-fang, ZHANG Yu-zhong, REN Li-wei, et al.   

  1. Yanjing College, Capital Medical University, Beijing 101300, China
  • Received:2011-04-28 Revised:1900-01-01 Published:2011-09-25 Online:2011-09-25

PDF (PC)

被引次数

8

摘要： 目的探讨地塞米松对内毒素休克大鼠脑组织细胞因子含量的影响。方法54 只健康成年Sprague-Dawley 大鼠分为假手术组、脂多糖组和地塞米松组，后两组静脉注射脂多糖8 mg/kg，地塞米松组于造模成功后10 min 静注地塞米松5 mg/kg。动态监测血压。分别于造模后1 h、3 h、6 h 用ELISA法检测脑组织中白细胞介素(IL)-4、IL-10 和肿瘤坏死因子(TNF)-α的含量。结果脂多糖组与假手术组比较，随着血压的下降，脑组织中IL-4、IL-10 浓度减少(P<0.01)，TNF-α增加(P<0.01)；与脂多糖组比较，地塞米松组TNF-α含量减少(P<0.01)，IL-4、IL-10 含量升高(P<0.01)。结论地塞米松能调节脂多糖诱导的内毒素休克大鼠脑内炎性因子，发挥脑保护作用。

关键词: 内毒素休克, 脑损伤, 地塞米松, 细胞因子, 大鼠

Abstract: Objective To explore the effect of the dexamethasone on the levels of cytokine in the brain of endotoxin shock rats. Methods 54 Sprague-Dawley rats were randomized to the sham group, LPS group (lipopolysaccharide 8 mg/kg, i.v.) and DEX group (dexamethasone 5 mg/kg, i.v., in addition). The blood pressure was measured dynamically. The contents of interleukin (IL)-4, IL-10 and tumor necrosis factor (TNF)-α were detected with ELISA 1 h, 3 h, 6 h after shock. Results As the occurrence and development of shock, blood pressure went down gradually, IL-4 and IL-10 decreased (P<0.01), and TNF-α increased (P<0.01) in LPS group, while the TNF-α decreased (P<0.01), the IL-4 and IL-10 increased (P<0.01) in the DEX group compared with the LPS group. Conclusion Dexamethasone may protect the brain from the endotoxin shock injury in rats through regulating the inflammatory factor.

Key words: endotoxin shock, brain injury, dexamethasone, cytokine, rats