《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (5): 426-428.

• 论文 • 上一篇    下一篇

肥胖大鼠心肌细胞内游离钙浓度的变化

郝艳坤，何志鹏，魏韬   

  1. 牡丹江医学院机能教研室，黑龙江省高校肿瘤疾病防治重点实验室，黑龙江牡丹江市 157011。
  • 收稿日期:2011-01-06 修回日期:1900-01-01 出版日期:2011-05-25 发布日期:2011-05-25

Change of Intracellular Calcium in Myocardial Cell of Obesity Prone Rats

HAO Yan-kun, HE Zhi-peng, WEI Tao.   

  1. Department of Animal Experiment, Mudanjiang Medical Colledg, Mudanjiang 157011, Heilongjiang, China
  • Received:2011-01-06 Revised:1900-01-01 Published:2011-05-25 Online:2011-05-25

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3

摘要： 目的探讨肥胖大鼠心肌细胞内钙调节机制。方法60 只健康雄性SD 大鼠高脂饲料喂饲10 周后，筛选出肥胖易感(OP)大鼠，基础组大鼠和肥胖抵抗(OR)大鼠，每组15 只。测量各组大鼠体脂肪含量，检测血脂水平。采用酶法分离心肌细胞，Fluo-3/AM负载后采用激光共聚焦扫描显微镜观察各组大鼠心肌[Ca2+]i对KCl 除极及咖啡因诱导的反性。结果与基础组大鼠和OR大鼠比较，OP 大鼠睾周脂肪、肾周脂肪、网膜脂肪、体脂比升高(P<0.05)，血清总胆固醇、三酰甘油和低密度脂蛋白水平升高(P<0.05)，心室肌[Ca2+]i的升高幅度降低(P<0.05)。结论肥胖心肌细胞[Ca2+]i在KCl 除极后及咖啡因诱导后升高幅度发生改变，可能是肥胖者心律失常发生机制之一。

关键词: 肥胖, 细胞内钙, 心律失常, 激光共聚焦扫描显微镜, 大鼠

Abstract: Objective To study the intracellular calcium ([Ca2+ ]i) in myocardial cell of obesity rats induced by high-fat diet. Methods Male Sprague-Dawley (SD) rats were divided into obesity resistant (OR, n=15), normal (Nor, n=15) and obesity prone (OP, n=15) group after fed with high-fat diet for 10 weeks. Their body fat and serum lipids were measured. Myocardial cells were isolated with Langendorff perfusion and [Ca2+]i was measured with calcium indicator Fluo-3/AM and laser scanning confocal microscope after KCl depolarization and caffeine- induced. Results Compared with those in Nor and OR rats, the epididymal fat, perirenal fat, omental fat and body fat increased in OP rats (P<0.05), as well as the the level of total cholesterol, triglyceride and low density lipoprotein (P<0.05); the vary of [Ca2+]i elevation and restoration were lower (P<0.05). Conclusion The vary of [Ca2+ ]i elevation decreases in OP rats after KCl depolarization and caffeine-induced, that may associated with arrhythmia in obesity rats.

Key words: obesity, intracellular calcium, arrhythmia, laser scanning confocal microscope, rats