《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (3): 235-239.

• 论文 • 上一篇    下一篇

吡咯喹啉醌对神经细胞氧糖剥夺损伤的保护作用及其机制

郭青，吕国枫   

  1. 大连医科大学运动医学教研室，辽宁大连市 116044。
  • 收稿日期:2010-12-14 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 吕国枫

Protective Effect of Pyrroloquinoline Quinine on Oxygen and Glucose Deprivation Injury in Nuro2A Cells

GUO Qing, Lü Guo-feng.   

  1. Department of Sport Medicine, Dalian Medical University, Dalian 116044, Liaoning, China
  • Received:2010-12-14 Revised:1900-01-01 Published:2011-03-25 Online:2011-03-25

PDF (PC)

摘要： 目的研究吡咯喹啉醌（PQQ）的神经保护作用及其可能机制。方法利用不同浓度PQQ预处理Neuro2A细胞后建立氧糖剥夺模型。复氧后6 h，观察Neuro2A细胞的细胞形态、存活率、凋亡率以及活性氧(ROS)和还原型谷胱甘肽(GSH)的浓度。结果Neuro2A细胞形态出现严重损伤；经0.4、0.8、1.6、3.2、6.4、12.8、25.6 μmol/L的PQQ预处理，细胞损伤减轻；细胞存活率随PQQ浓度升高呈逐渐增高趋势；经6.4 μmol/L和12.8 μmol/L的PQQ预处理，细胞凋亡减少，细胞内ROS生成减少，GSH水平增高（P＜0.05）。结论PQQ对氧糖剥夺神经细胞损伤有一定的保护作用，这一作用可能是通过减轻氧化应激反应，降低细胞凋亡率实现的。

关键词: 吡咯喹啉醌, 氧糖剥夺, 凋亡, Neuro2A细胞, 氧化应激

Abstract: ObjectiveTo investigate the neuroprotective effects of pyrroloquinoline quinine (PQQ) on oxygen and glucose deprivation (OGD) in cultured rat neuroblastoma cells Neuro2A and the possible mechanisms involved. MethodsCultured rat neuroblastoma cells Neuro2A were pretreated or not pretreated with increasingly concentrations of PQQ, were exposed to 2 h combined OGD in an anaerobic chamber followed by reoxygenation of 6 h. The results on cellular morphology, cell viability，apoptotic cells, reactive oxygen species (ROS), and glutathione (GSH) were compared between OGD and PQQ group. ResultsPretreatment of PQQ (0.4, 0.8, 1.6, 3.2, 6.4, 12.8 μmol/L) increased the cell viability (P＜0.05) and GSH level, decreased the contents of ROS and the percentage of apoptotic cells in cortical neurons exposure to OGD (P＜0.01). ConclusionPQQ exhibits remarkable protection against hypoxia/reoxygenation injury in Neuro2A cells, which may be associated to the inhibiting the oxidative stress and subsequent apoptosis.

Key words: pyrroloquinoline quinine, oxygen and glucose deprivation, apoptosis, Nuro2A cells, oxidative stress