《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (4): 307-309.

• 论文 • 上一篇    下一篇

针康法对局灶性脑缺血大鼠学习记忆能力及海马基质细胞衍生因子-1α mRNA的影响

赵振峰1,2，王芳2，刘宏光2，李洪梅2，唐强2   

  1. 1.哈尔滨医科大学附属第五医院康复医学科，黑龙江大庆市 163000；2.黑龙江中医药大学，黑龙江哈尔滨市 150040。
  • 收稿日期:2011-03-16 修回日期:1900-01-01 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 唐强

Effect of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Learning and Memory and Hippocampus Stromal Cell-derived Factor-1α mRNA in Rats after Focal Cerebral Ischemia

ZHAO Zhen-feng, WANG Fang, LIU Hong-guang, et al.   

  1. Department of Rehabilitation Medicine, the 5nd Hospital affiliated to Harbin Medical University, Harbin 163000, Heilongjiang, China
  • Received:2011-03-16 Revised:1900-01-01 Published:2011-04-25 Online:2011-04-25

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摘要： 目的探讨针康法对局灶性脑缺血大鼠学习记忆能力及海马基质细胞衍生因子1α(SDF-1α) mRNA的影响。方法将60只大鼠随机分为正常对照组组 (n=6)、模型组 (n=18）、针康组 (n=18)和康复组 (n=18)。采用光化学法诱导局灶性脑缺血模型，将模型组、针康组和康复组再分为3 d、7 d、14 d 3个亚组。术后24 h对针康组进行头穴丛刺结合水迷宫训练干预，康复组仅进行水迷宫训练，模型组不给予任何干预。采用Y-型迷宫评价大鼠的学习记忆能力，反转PCR(RT-PCR)法观察各组大鼠海马组织中SDF-1α mRNA表达情况。结果术后各时间点，正常对照组、针康组、针康组大鼠学习能力优于模型组(P＜0.05)，与康复组比较，针康组术后3 d无显著性差异(P>0.05)；术后7 d、14 d有显著性差异(P＜0.05)；与正常对照组比较，模型组、针康组和康复组均有显著性差异(P＜0.05)；与模型组比较，针康组、康复组SDF-1 mRNA均有显著性差异(P＜0.05)；与康复组相比，针康组SDF-1 mRNA表达较高，术后3 d、7 d显著性差异(P＜0.05)，14 d无显著性差异(P＞0.05)。结论针康法能上调局灶性脑缺血大鼠海马SDF-1α mRNA，这可能是其促进局灶性脑缺血大鼠学习记忆能力的提高机制之一。

关键词: 脑缺血, 针康法, 学习记忆, 基质细胞衍生因子-1α mRNA, 大鼠

Abstract: ObjectiveTo investigate the effect of cluster needling of scalp acupuncture combined with rehabilitation(Tang's Approach) on learning and memory and hippocampus SDF-1α mRNA in rats after focal cerebral ischemia.Methods60 rats was randomly divided into 4 groups: the control group (n=6), the model group (n=18), the acupuncture with rehabilitation group (n=18) and rehabilitation group (n=18). Photochemical activity was adopted to induce focal cerebral ischemia model. The acupuncture with rehabilitation group, the model group and rehabilitation group was divided into 3 subgroups by 3,7,14 days. 24 hours after molded, the cluster needle of scalp point with Water maze training was used in the acupuncture with rehabilitation group, Water maze training was used in the rehabilitation group, but the model group did not intervene. The learning and memory in rats was evaluated by Y-maze, and the expression of hippocampus SDF-1α mRNA was observed by RT-PCR method on 3th, 7th, 14th day after infarction.ResultsOn 3th, 7th, 14th day after infarction, the scores of learning and memory in other groups was less than the control group(P<0.05), but the learning and memory in the acupuncture with rehabilitation group had been restored, and the difference was statistically significant compared with rehabilitation group (P＜0.05) on 7th, 14th day after infarction; the expression of hippocampus SDF-1α mRNA has been risen in the acupuncture with rehabilitation group compared with the rehabilitation group (P＜0.05）, but the difference was not significant on 14th day after surgery(P＞0.05）.ConclusionThe hippocampus SDF-1α mRNA after focal cerebral ischemia increases by the cluster needle of scalp point with Water maze training, which may be one of the mechanisms to improve the learning and memory by Tang's Approach after focal cerebral ischemia.

Key words: cerebral ischemia, Tang's Approach, learning and memory, stromal cell-derived factor-1α mRNA, rats