《中国康复理论与实践》

《中国康复理论与实践》 ›› 2011, Vol. 17 ›› Issue (4): 313-315.

• 论文 • 上一篇    下一篇

针康法对免疫抑制大鼠细胞免疫功能的影响

朱志中1，唐强2,3，颜培宇3   

  1. 1.天津市环湖医院康复医学科，天津市 300060；2.黑龙江中医药大学附属第二医院，黑龙江哈尔滨市 150001；3.黑龙江中医药大学，黑龙江哈尔滨市 150004。
  • 收稿日期:2011-03-16 修回日期:1900-01-01 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 唐强

Effect of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Cellular Immunity of Immunosuppressive Rats

ZHU Zhi-zhong, TANG Qiang, YAN Pei-yu.   

  1. Tianjin Huanhu Hospital, Tianjin 300060, China
  • Received:2011-03-16 Revised:1900-01-01 Published:2011-04-25 Online:2011-04-25

PDF (PC)

摘要： 目的观察针康法对免疫抑制大鼠模型血清白细胞介素-4(IL-4)及T细胞亚群的影响。方法将40只健康SD大鼠(雄性)体重200～250 g，完全随机分为5组：正常组、模型组、康复组、针康组、针刺组，每组8只。除正常组和模型组外，其他各组分别给予针刺或康复治疗，每天1次，治疗10 d后，取大鼠血清。用放射免疫法检测血清中IL-4含量及外周血CD4+ T细胞和CD8+ T细胞的含量。结果模型组血清IL-4水平升高，与正常组比较有显著性差异(P＜0.05)。康复治疗和针康法都可降低免疫抑制大鼠血清IL-4水平，与模型组比较有显著性差异(P＜0.05)；模型组大鼠CD4+ T细胞水平下降，与正常组比较有显著性差异(P＜0.05)；经针刺和康复治疗后，针刺组和针康组大鼠外周血CD4+ T细胞水平明显升高，与模型组比较有非常显著性差异(P＜0.01)。实验各组CD8+ T细胞水平于治疗前后无显著性差异(P>0.05)。结论针康法能降低大鼠血清中IL-4的含量，显著提高免疫抑制大鼠低下的CD4+T细胞水平，提高机体的细胞免疫功能。

关键词: 免疫抑制, 针康法, 白细胞介素-4, T细胞亚群, 大鼠

Abstract: ObjectiveTo observe the effect of cluster needling of scalp acupuncture combined with rehabilitation(Tang's Approach) on interleukin-4 (IL-4), T lymphocyte subsets of immunosuppressive model rat.Methods40 healthy SD rats (male, 200～250 g) was divided into 5 groups randomly: normal group (NG), model group (MG), acupuncture group(AG), rehabilitation group(RG), and acupuncture with rehabilitation group(ARG), 8 rats in each group. Each group was treated by acupuncture or rehabilitation except the model or normal group, once a day for 10 d. Radioimmunoassay and malondialdehydein (MDA) technique were used to test the contents of IL-4 and T lymphocyte subsets in each group. ResultsThe contents of IL-4 of immunosuppressive rats can decrease significantly in RG and ARG (P＜0.05). The level of CD4+ T lymphocyte increased more significantly in AG and RAG than in MG after the treatment (P＜0.01). ConclusionTang's Approach can decrease the contents of IL-4 and improve the level of CD4+ T lymphocyte in rats.

Key words: immunosuppression, Tang's Approach, interleukin-4, T lymphocyte subsets, rat