《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (01): 35-37.

• 基础研究 • 上一篇    下一篇

缺氧缺血对新生乳鼠脑组织的损伤作用

温林豹1;刘伟2a;陈刚2a;赵丽辉2b;杨小朋2a;孙振柱2b;迟广明2a   

  1. 1.新疆医科大学研究生院，新疆乌鲁木齐市 830054;2.新疆维吾尔自治区人民医院，a.神经外科;b.病理科，新疆乌鲁木齐市 830001
  • 收稿日期:2008-08-08 修回日期:2008-11-24 出版日期:2009-01-01 发布日期:2009-01-01
  • 通讯作者: 陈刚

Pathological Study of Perinatal Hypoxic-Ischemic Brain Damage in Rats

WEN Lin-bao, LIU Wei, CHEN Gang, et al   

  1. Xinjiang Medical University, Urumqi 830054, Xinjiang, China
  • Received:2008-08-08 Revised:2008-11-24 Published:2009-01-01 Online:2009-01-01

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摘要： 目的 观察缺氧缺血对新生乳鼠脑组织病理学改变。方法 70只健康7日龄Wistar新生乳鼠，结扎后离断左侧颈总动脉，置于37.0 ℃、8% O2+92% N2缺氧箱2 h后取出，分别于7 d、14 d、30 d处死取脑，HE染色、免疫组化、透射电镜观察脑组织病理学改变。结果 HE染色：7 d后结扎侧脑室周围可见神经细胞排列紊乱，部分神经细胞变性坏死，细胞肿胀，炎细胞浸润；30 d结扎侧脑室周围多个囊腔形成；免疫组化染色：结扎侧脑室周围髓鞘碱性蛋白（MBP）阳性细胞数随时间延长逐渐减少，而胶质纤维酸性蛋白（GFAP）阳性细胞数随时间延长逐渐增多，部分染色包绕囊腔，局部染色聚集；电镜：7 d可见细胞形态尚规则、水肿，双层结构不清，部分线粒体空泡样变，细胞核染色质浓缩；30 d可见细胞形态不规则，部分核膜破损，核内容物外溢。结论 新生乳鼠一侧颈总动脉结扎结合缺氧的方法可造成结扎侧脑室周围细胞水肿，变性坏死，软化灶形成。

关键词: 缺氧缺血性脑病, 脑室周围白质软化, 大鼠

Abstract: Objective To investigate the pathological changes in neonatal rats after hypoxic-ischemic insults. Methods 70 normal 7-day-old Wistar rats were divided into two groups randomly: hypoxic-ischemic and control group. The left carotid arteries were permanently ligated and cut in hypoxic-ischemic group. All the animals were exposed hypoxia (92% N2, 8% O2) for 2 h in a constant thermal environment. All the brain tissues were observed with electronmicroscopy, HE and immunohistochemistry stain 7 d, 14 d and 30 d after injury. Results HE stain showed that neurocytes arranged disorder in the side of the carotid ligation 7 d after injury. Some degenerated neurocytes, swelled cells and inflammatory cell infiltration also visualized. Many capsular spaces were observed in the side of the carotid ligation 30 d after injury. Immunohistochemistry staining showed Myelin basic protein (MBP) positive neurocytes gradually decreased, and glia fibrillary acidic protein (GFAP) positive neurocytes gradually inreased in the periventricular zone with the time lapsed. It was observed that the neurocytes were edema, many mitochondrias were vacuolus, caryotins were condensed under transmission electron microscope 7 d after operation. The morphocytology of the neurocytes were irregulated obviously, part of nuclear membranes were broken down with the content leakaged 30 d after operation. Conclusion The characteristics of periventricular leukomalacia can be developed after hypoxic-ischemic insults in the rats.

Key words: hypoxic-ischemic encephalopathy, periventricular leukomalacia, rats