《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (07): 389-390.

• 专题 • 上一篇    下一篇

促甲状腺素释放激素类似物YM14673对大鼠脑外伤后脑水肿及血脑屏障通透性的影响

张绍东; 翟晶; 张辉; 张家瑾   

  1. 北京市神经外科研究所 北京市 100050
  • 收稿日期:2004-05-17 出版日期:2004-07-25 发布日期:2004-07-25

Effect of thyrotrophin-releasing hormone analogue, YM14673,on the blood-brain barrier and brain edema after head trauma in rats

ZHANG Shao-dong, ZHAI Jing,ZHANG Hui, et al   

  1. Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2004-05-17 Published:2004-07-25 Online:2004-07-25

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摘要： 目的观察促甲状腺素释放激素(TRH)类似物YM14673对脑外伤后血脑屏障的影响。方法制作大鼠急性脑外伤模型,外伤前静脉注入伊文氏蓝。大鼠分为假手术组、生理盐水组、给药组Ⅰ(0.1mg/kg)、给药组Ⅱ(1mg/kg)。伤后24h处死,测定脑水含量及脑组织和血浆中的伊文氏蓝含量。结果大鼠脑外伤后,生理盐水组脑组织脑水含量明显高于假手术组(P<0.01),遭受直接打击的左侧半球脑水含量明显高于右半球(P<0.01)。经药物YM14673处理后,左右半球的脑水含量均下降(P<0.05),而不同剂量治疗组之间脑水含量无显著性差异(P>0.05)。急性脑外伤后,脑组织中伊文氏蓝含量明显升高,YM14673对其没有影响。结论YM14673可减轻急性脑外伤引起的脑水肿,但对血脑屏障通透性没有影响。

关键词: 促甲状腺素释放激素(TRH), YM14673, 脑外伤, 血脑屏障, 脑水肿

Abstract: Objective To study the effect of thyrotrophin releasing hormone (TRH) analogue, YM14673, on the brain edema and blood brain barrier after brain injury in rat.Methods The model of brain injury of rats was built by Feeney's methods. The Evans blue solution had been injected i.v. into the rats before the models were made. The rats were divided into four groups: normal, treated with saline, treated with YM14673(Ⅰ:0.1mg/kg and Ⅱ:1mg/kg). The water content in brain was measured 24 h after brain injured. The concentration of Evans blue in brain tissue and blood was measured with fluorometry.Results The rats treated with saline after traumatic injury showed significantly high water content compared with normal group(P<0.01)and the water content of the left hemisphere, which was hit straightly, was higher significantly than that of the right global(P<0.01). The brain water content decreased in the rats treated with YM14673 in all global(P<0.05).There was no significant different between the treated group Ⅰ and groupⅡ.After brain injury, the concentration of Evans blue in brain tissue showed a higher level contrasted with normal group. YM14673 did not influence the concentration of Evans blue in brain tissue. Conclusion YM14673 can decrease the brain edema after brain injury but it cannot decrease the permeability of blood brain barrier.

Key words: thyrotrophin-releasing hormone (TRH), YM14673, brain trauma, blood-brain barrier, brain edema