《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (11): 647-648.

• 专题 • 上一篇    下一篇

空间诱变致生长减慢CHO(dhfr-)细胞株的特性

李红艳; 徐梅; 向青; 房青; 徐波; 高福云; 刘国铃; 唐劲天   

  1. 中日友好医院临床医学研究所生化及分子生物学研究室 北京市 100029
  • 收稿日期:2004-10-13 出版日期:2004-11-25 发布日期:2004-11-25

Characteristic of growth decreased CHO(dhfr-) cell line mutated by outer space

LI Hong-yan, XU Mei, XIANG Qing,et al   

  1. Department of Biochemistry and Molecular Biology, China-Japan Friendship Clinical Medicine Institute, Beijing 100029, China
  • Received:2004-10-13 Published:2004-11-25 Online:2004-11-25

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摘要： 目的了解空间环境下生物工程细胞CHO(dhfr-)在形态、生长和周期等方面的变化。方法将CHO(dhfr-)生物工程细胞株搭载于我国第18颗返回式卫星,返地的细胞在倍增之前进行单克隆化。从中随机选取4株细胞,经5代培养确定一株生长速度最慢的细胞株,利用光镜、MTT法、FCM分析法观察细胞生长特性。结果返地后的CHO (dhfr-)细胞经过单克隆化、扩增,获得159个细胞株。经空间诱变后的细胞出现细胞形态改变、生长增殖速度减慢、G1期细胞明显增多。结论生物工程CHO(dhfr-)细胞经空间诱变可引起生长特性的改变,为筛选优化的生物工程制药细胞提供可能。

关键词: CHO(dhfr-)细胞, 空间, 生长特性

Abstract: ObjectiveTo investigate the changes of the morphology, growth and cycle of CHO(dhfr-) cells after space flight.MethodsCHO(dhfr-) cells were carried in the No.18 recoverable satellite and monocloned harvesting cells before multiplying. 4 cell lines were selected randomly,and the growth characteristics of the most slowly growing one at the fifth passage was observed by methods of MTT and FCM as well as the cells' shape.Results159 cell strains were obtained after monocloning and multiplying. The cells' morphology changes, growth speed decrease and the number of G1 phase increased markedly.ConclusionSpace flight induced morphological changes of cells and it is impossible to screen out finer bioengineering cells.

Key words: CHO(dhfr-) cell, outer space, growth characteristic