《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (01): 29-31.

• 专题　药物康复 • 上一篇    下一篇

灯盏细辛对大鼠缺血性脑损伤代谢物质的影响

陈文1学; 刘华2; 廖维靖2; 岳勇1; 邓风1   

  1. 1.中国科学院武汉物理与数学研究所,波谱与原子分子物理国家重点实验室,湖北武汉市 430071;2.武汉大学中南医院,湖北武汉市 430071
  • 收稿日期:2006-10-18 出版日期:2007-01-01 发布日期:2007-01-01

Effects of Herba Erigerontis on Metabolites in Cerebral Ischemia Rats

CHEN Wen-xue, LIU Hua, LIAO Wei-jing, et al   

  1. State Key Laboratory of Magnetic Resonance and Atomic Molecular and Physics, Wuhan Institute of Physics and Mathematics, theChinese Academy of Sciences, Wuhan 430071, Hubei, China
  • Received:2006-10-18 Published:2007-01-01 Online:2007-01-01

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摘要： 目的观察大鼠缺血性脑损伤对大、小脑波谱的影响以及灯盏细辛对大鼠缺血性脑损伤代谢物质变化的影响。方法18只雄性Wistar大鼠,体重150～200g,随机分成正常对照组(n=6)、缺血组(n=6)、灯盏细辛治疗组(n=6)。采用大脑中动脉栓塞法(MCAO)制作大鼠急性脑缺血损伤模型,缺血3h后,恢复血供或灯盏细辛治疗。采用离体高分辨魔角旋转波谱(HRMAS1HNMR)技术观察大、小脑波谱中胆碱(Cho)、肌酸(Cre)、N-乙酰天门冬氨酸(NAA)、γ-氨基丁酸(GABA)、谷氨酸(Glu)、天冬氨酸(Asp)等共振峰的变化。结果除Cho、Glu、Asp升高,大鼠急性脑缺血时大多数代谢物浓度均明显低于正常对照组,经灯盏细辛治疗后其代谢物的浓度会有所增加。结论缺血严重影响大、小脑神经元的存活和物质代谢,灯盏细辛对大鼠缺血性脑损伤有明显的保护作用。

关键词: 核磁共振, 魔角旋转, 模式识别, 脑缺血, 灯盏细辛, 大鼠

Abstract: ObjectiveTo study effect of Herba Erigerontis on metabolites in cerebral ischemia rats with high-resolution magic angle spinning 1H nuclear magnetic resonance spectroscopy(HR MAS 1H NMR). Methods18 male Wistar rats, weighing 150～200 g, were randomly divided into 3 groups such as normal (n=6), ischemia (n=6), Herba Erigerontis treatment (n=6). Rat ischemia model was established with middle cerebral artery occlusion (MCAO), reperfusing or dosed with Herba Erigerontis after 3 h ischemia. The metabolites, including N-acetylaspartate (NAA), creatine (Cre), choline (Cho), glutamate (Glu), and aspartate (Asp) etc., of cerebra and cerebella was observed using HR MAS 1H NMR. ResultsExcluding Cho, Glu, Asp, the concentrations of most metabolites of rat brain during ischemia were significantly lower than that of normal rats, and could be increased after drug treatment. ConclusionHerba Erigerontis shows positive effect on metabolites in cerebral ischemia rats.

Key words: nuclear magnetic resonance spectroscopy, magic angle spinning, pattern recognition, cerebral ischemia, Herba Erigerontis, rats