《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (03): 238-239.

• 基础研究 • 上一篇    下一篇

血-脑屏障和血-神经屏障内皮细胞的比较研究

陈海波1; 陶沂1; 尹岭2; 丁素菊1; 刘式威2; 杜侃2   

  1. 1.第二军医大学长海医院神经内科,上海市 200433;2.解放军总医院神经信息中心,北京市 100853
  • 收稿日期:2006-10-31 出版日期:2007-03-01 发布日期:2007-03-01

Difference of Endothelial Cell in Ultrastructure,Markers and Permeability between Blood-Brain Barrier and Blood-Nerve Barrier

CHEN Hai-bo,TAO Yi, YIN Ling, et al   

  1. Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2006-10-31 Published:2007-03-01 Online:2007-03-01

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被引次数

4

摘要： 目的研究血-脑屏障与血-神经屏障内皮细胞在超微结构、通透性及标记物表达方面的差异。方法取大鼠前额叶皮层和坐骨神经。用电镜观察内皮细胞的超微结构;用免疫组织化学的方法检测转铁蛋白受体(OX-26)和内皮屏障抗原(EBA)的表达及微血管周围纤维蛋白原外渗。结果电镜显示,血-脑屏障和血-神经屏障微血管均为紧密连接,但血-神经屏障吞饮小泡较血-脑屏障多(P<0.05)。免疫组织化学显示,血-神经屏障OX-26和EBA阴性表达,微血管周围可见微量纤维蛋白原外渗;血-脑屏障OX-26和EBA均强阳性表达,微血管周围未见纤维蛋白原外渗。结论血-脑屏障与血-神经屏障内皮细胞虽然均为紧密连接,但两者在吞饮小泡数量、标记物表达及通透性等方面均存在差异。

关键词: 血-脑屏障, 血-神经屏障, 超微结构, 免疫组织化学

Abstract: Objective To investigate the difference of endothelial cell (EC) in ultrastructure,markers and permeability between blood-brain barrier (BBB) and blood-nerve barrier (BNB).MethodsThe rat brain cortex and sciatic nerve were removed.Ultrastructure of endothelial cell was observed with electron microscopy.Employing immunohistochemical (IHC)staining,the relative distribution was determined for the OX-26,endothelial barrier antigen (EBA)and extravasated fibrinogen around microvessels.ResultsMicrovessels both in BBB and in BNB did share the same tight junctions.However,BNB had a significantly larger number of vesicles than that of BBB.Microvessels in BNB did not express the OX-26 and EBA,but staining weakly positive for fibrinogen,while microvessels in BBB showed strong positive for OX-26 and EBA,perivascular staining of fibrinogen had not been seen.ConclusionTight junctions were present in both BBB and BNB endothelial cell,but there was a significant difference between them in the number of the pinocytotic vesicles,markers expression and permeability.

Key words: blood-brain barrier, blood-nerve barrier, ultrastructure, immunohistochemistry