《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (05): 376-377.

• 专题 • 上一篇    下一篇

腺病毒转染一氧化氮合酶基因抑制大鼠静脉桥血管内膜增生

李铁岭; 张金山; 郭水龙; 范利   

  1. 解放军总医院干部诊疗科 北京市 100853
  • 收稿日期:2006-04-24 出版日期:2006-05-25 发布日期:2006-05-25

Transfection of Nitric Oxide Synthase Gene with Adenovirus Can Inhibit the Intimal Hyperplasia of Venous Autografts

LI Tie-ling,ZHANG Jin-shan, GUO Shui-long, et al   

  1. General Hospital of PLA, Beijng 100853, China
  • Received:2006-04-24 Published:2006-05-25 Online:2006-05-25

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被引次数

3

摘要： 目的探讨转染一氧化氮合酶(NOS)基因对自体移植静脉内膜增生的影响。方法制作20只自体颈静脉腹主动脉移植Wistar大鼠模型,实验组、对照组各10只。移植前,实验组血管行腺病毒介导的eNOS溶液浸泡,对照组仅行空载腺病毒溶液浸泡。术后2周取出移植血管,利用病理学、免疫组织化学、RTPCR检测移植血管内膜厚度、管腔狭窄度、内膜血管平滑肌细胞(VSMC)数及PCNA阳性表达、血管eNOS mRNA表达情况。结果实验组移植血管内膜厚度、管腔狭窄度、VSMC数及PCNA阳性表达均较对照组明显减小或减少,而eNOS mRNA表达则明显增加(均P<0.01)。结论移植血管转染NOS基因可有效抑制移植静脉内膜的增生。

关键词: 一氧化氮合酶(NOS), 再狭窄, 静脉桥血管, 基因治疗

Abstract: ObjectiveTo investigate the effects of nitric oxide synthase (NOS) genetic transfection on the intimal hyperplasia of venous autografts. MethodsThe external jugular veins were autografted into abdominal aorta arteries in 20 Wistar rats, which were divided evenly into experimental or control groups. The transplanted veins of experimental group were immersed in the adenovirus-mediated eNOS gene solution for 15 minutes just before anastomosis. The transplanted vascular samples were taken out 2 weeks after operation. The intimal thickness(IH), degree of restenosis(DR), expression of PCNA and NOS mRNA were determined with histology and transcription polymerase chain reaction (PCR). ResultsThe IH, DR and PCNA decreased, while the expression of eNOS mRNA increased comparing with control group(P<0.01). ConclusionTransfection of NOS gene can inhibit the intimal hyperplasia of venous autografts.

Key words: nitric oxide synthase (NOS), restenosis, vascular grafts, gene therapy