《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (05): 420-422.

• 临床研究 • 上一篇    下一篇

Analysis of Gene Expression Pattern of Lumbar Intervertebral Disc Degeneration in Human

  

  • 收稿日期:2006-03-08 出版日期:2006-05-25 发布日期:2006-05-25

Analysis of Gene Expression Pattern of Lumbar Intervertebral Disc Degeneration in Human

HU Ming; MA Yuan-zheng; FENG Hui-cheng; CHEN Xing; CHAI Xiao-jun; PENG Wei; LI Hong-wei   

  1. The Orthopaedic Department; PLA309 Hospital; Beijing 100091
  • Received:2006-03-08 Published:2006-05-25 Online:2006-05-25

Abstract: ObjectiveTo investigate the gene expression changes in normal and degeneration lumbar intervertebral disc in humans, providing information for clinical. MethodsThe PCR products of 4096 human genes were spotted onto a kind of chemical-material-coated-glass slides. The total RNAs were isolated from the tissues. Both the mRNAs from the degeneration and normal lumbar intervertebral disc in humans were reversely transcribed to the cDNAs, which used as the hybridization probes with the incorporations of fluorescent dUTP. The mixed probes were then hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and analyzed with computer image analysis. ResultsAmong the 4096 targets, there were 706 genes whose expression levels differed between the degeneration and normal lumbar intervertebral disc in all cases, comprising 298 up-regulated and 358 down-regulated ones. ConclusionDNA microarray technology is an effective technique in screening for differently expressed genes between the degeneration and normal lumbar intervertebral disc. Cell apoptosis plays an important role in the process of lumbar intervertebral disc degeneration.

Key words: intervertebral disc degeneration, DNA microarray, gene expression pattern