《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (04): 314-315.

• 专题 • 上一篇    下一篇

地黄低聚糖抗过氧化氢诱导的脂肪间充质干细胞凋亡的保护作用

王玉红1,2;王舒2; 张琰琴1; 王磊1; 秦宇红3; 陈光辉1   

  1. 1.解放军总医院心血管内科,北京市 100853;2.北京军区总医院263临床部,北京市 101149;3.解放军总医院第一附属医院急诊科,北京市 100037
  • 收稿日期:2007-12-13 出版日期:2008-04-01 发布日期:2008-04-01

Effect of Rehmannia Glutinosa Oligosaccharide on Apoptosis of Human Adipose Tissue-derived Mesenchymal Stromal Cells Induced by Hydrogen Peroxide

WANG Yu-hong, WANG Shu, ZHANG Yan-qin, et al   

  1. The Department of Cardiology, the General Hospital of PLA, Beijing 100853, China
  • Received:2007-12-13 Published:2008-04-01 Online:2008-04-01

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摘要： 目的观察地黄低聚糖对H2O2诱导的人脂肪间充质干细胞凋亡的影响。方法将体外培养的人脂肪间充质干细胞随机分成正常对照组(N组,无特殊处理)、H2O2组(H组)和地黄低聚糖组(R组)。H组加入终浓度0.1mmol/L的H2O2;R组加入终浓度0.2g/L的地黄低聚糖和终浓度0.1mmol/L的H2O2。三组的干预时间分别为1h、6h和24h,采用流式细胞仪检测细胞凋亡率。结果干预1h、6h和24h后,H组脂肪间充质干细胞凋亡率明显高于N组和R组(P<0.01);R组脂肪间充质干细胞凋亡率明显低于H组(P<0.01)。结论地黄低聚糖可减轻H2O2诱导的人脂肪间充质干细胞凋亡。

关键词: 脂肪间充质干细胞, 地黄低聚糖, 凋亡, 过氧化氢

Abstract: Objective To investigate the influence of rehmannia glutinosa oligosaccharide(RGOs)on apoptosis of human adipose tissue-derived mesenchymal stromal cells(ADMSCs)induced by hydrogen peroxide(H2O2).Methods Cultured human ADMSCs were randomly divided into three groups as the normal group(group N,without any treatment),H2O2 group(group H,treated with 0.1 mmol/L H2O2),and RGOs group(group R,treated with 0.2g/L RGOs plus 0.1 mmol/L H2O2).After 1 h,6 h and 24 h,morphological changes of ADMSCs apoptotic were observed,the apoptotic rate was determined by flow cytometry.Results After 1 h,6 h and 24 h,the apoptotic rate of group H was significantly higher than that of the group N and group R(P<0.05),and the apoptotic rate of group H was significantly higher than that of the group R(P<0.05).Conclusion RGOs can attenuate apoptosis of human ADMSCs induced by H2O2.

Key words: adipose tissue-derived mesenchymal stromal cells, rehmannia glutinosa oligosaccharide, apoptosis, hydrogen peroxide