《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (05): 433-434.

• 基础研究 • 上一篇    下一篇

通心络对糖尿病大鼠脑缺血后神经生长因子及脑源性神经营养因子表达的影响

田沈1; 张朝东2; 康志伟1   

  1. 1.中国医科大学第四临床医院神经内科,辽宁沈阳市 110032;2.中国医科大学第一临床医院神经内科,辽宁沈阳市 110001
  • 收稿日期:2007-12-19 出版日期:2008-05-01 发布日期:2008-05-01

Effects of Tongxinluo on Nerve Growth Factor and Brain Derived Neurotrophic Factor after Cerebral Ischemia in Diabetic Rats

TIAN Shen, ZHANG Chao-dong, KANG Zhi-we   

  1. Departmentof Neurology, The First Affiliated Hospital, China Medical University, Shenyang 110001,Liaoning, China
  • Received:2007-12-19 Published:2008-05-01 Online:2008-05-01

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摘要： 目的探讨通心络对改善局灶性脑缺血糖尿病大鼠的神经功能的作用,并探讨其分子机制。方法线栓法复制糖尿病大鼠大脑中动脉栓塞模型,干预组通心络胶囊1.0g/kg·d连续灌胃。进行神经功能缺损评分;采用TUNEL染色计数脑缺血周边区神经细胞凋亡数,免疫组织化学方法检测神经生长因子(NGF)及脑源性神经营养因子(BDNF)的蛋白表达。结果干预组神经功能缺损评分低于对照组(P<0.05);缺血周边区TUNEL阳性细胞数均少于对照组(P<0.05);NGF、BDNF蛋白免疫阳性细胞多于对照组(P<0.05)。结论通心络可增加糖尿病脑缺血后缺血周边区NGF、BDNF蛋白的表达,拮抗神经细胞凋亡,改善神经功能。

关键词: 糖尿病, 脑缺血, 通心络, 凋亡, 神经生长因子(NGF), 脑源性神经营养因子(BDNF), 大鼠

Abstract: Objective To investigate the effects of Tongxinluo,a Chinese medicine,on neurological deficits,neuron apoptosis and the expression of nerve growth factor(NGF) and brain derived neurotrophic factor(BDNF) after cerebral ischemia in diabetic rats.Methods Wistar diabetic rats(n=36) were subjected to middle cerebral artery occlusion and randomly divided into 2 groups:control group(n=18) and Tongxinluo group(n=18).Tongxinluo group received Tongxinluo 1.0 g/kg·d after cerebral ischemia.Neurological severity scores,TUNEL staining and immunohistological assessments were performed to evaluate the effects.Results Compared with the control,the neurological score significantly improved(P<0.05),the expression of NGF and BDNF increased(P<0.05),and cell apoptosis decreased(P<0.05) in the tongxinluo group.Conclusion Tongxinluo can improve the neurological function through inhibition of neuron apoptosis and increasing the expression of NGF and BDNF after cerebral ischemia in diabetic rats.

Key words: diabetes, cerebral ischemia, Tongxinluo, apoptosis, nerve growth factor(NGF), brain derived neurotrophic factor(BDNF), rats