《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (07): 602-603.

• 专题　神经源性膀胱及不尿路管理 • 上一篇    下一篇

雌性大鼠尿道壁内横纹括约肌一氧化氮合酶分布的免疫组化研究

鞠彦合1,2; 邱志勇1,2; 廖利民1,2; 叶云龙1,2   

  1. 1.中国康复研究中心北京博爱医院泌尿外科,北京市 100068;2.首都医科大学康复医学院,北京市 100068
  • 收稿日期:2007-05-13 出版日期:2007-07-01 发布日期:2007-07-01

Nitric Oxide Synthase Expression in Intramural Striated Sphincter of Female Rats

JU Yan-he,QIU Zhi-yong, LIAO Li-min, et al   

  1. Department of Urology, Beijing Charity Hospital, China Rehabilitation Research Center, School of Rehabilitation, Capital Medical University, Beijing 100068, China
  • Received:2007-05-13 Published:2007-07-01 Online:2007-07-01

PDF (PC)

被引次数

6

摘要： 目的研究神经型一氧化氮合酶(nNOS)在大鼠尿道壁内横纹肌的分布情况。方法雌性Wistar大鼠4只,水合氯醛腹腔注射麻醉后,解剖膀胱和尿道全长标本,福尔马林固定,石蜡包埋,2只做平行尿道长轴的纵行切片,2只做垂直尿道的横行切片,常规HE染色、Mallory磷钨酸苏木素染色了解尿道的显微解剖。定位尿道横纹括约肌后局部进行nNOS免疫组化染色。结果雌性Wistar大鼠的尿道为一长约1.5～2.0cm的管状结构。其典型的组织结构由外向内依次为:环行横纹肌纤维层、环行平滑肌纤维层、纵行平滑肌纤维层、致密结缔组织层和上皮层。全尿道标本纵切面显示,尿道壁内横纹括约肌并不分布于尿道全长,而是仅环绕尿道的中下段。横切面显示尿道壁内横纹括约肌呈封闭环形,约5～10层不等,但并不是均匀分布,后壁横纹肌层较前壁为厚,层数稍多。nNOS免疫活性在部分尿道壁内横纹肌内呈强阳性表达。结论nNOS在部分尿道壁内横纹肌内呈强阳性表达,提示其对尿道壁内横纹肌可能起重要的调节作用。

关键词: 内横纹括约肌, 一氧化氮合酶, 尿道, 大鼠

Abstract: Objective To investigate the neuronal nitric oxide synthase (nNOS) distribution in intramural striated sphincter of female rats. Methods 4 female Wistar rats were anesthetized with chloral hydrate. The whole bladder and urethra specimen were harvested, fixed in formalin and imbedded in paraffin wax. Longitudinal and transverse sections were developed with 2 urethra respectively. Routine HE staining and Mallory phosphotungstic acid-hematoxylin staining were used for observation of urethral microstructure. nNOS distribution in intramural striated sphincter was determined with immunohistochemical staining. Results The urethras of female Wistar rat were tubular structure about 1.5～2.0 cm long. From outside inward,the structure included circular striated muscle layer,circular smooth muscle layer,longitudinal smooth muscle layer,dense connective tissue and epithelium. The longitudinal sections showed intramural striated muscle predominates the middle and distal third,not the whole urethral length. The transverse sections showed intramural striated muscle is closed ring shape,about 5～10 layers uneven distributed with more layers in the posterior wall than in the front wall. Strong positive expression of nNOS immunoreactivity was found in some intramural striated muscle fiber. Conclusion nNOS expresses in some intramural striated muscle fiber, surggesting nitric oxide may play an important role on intramural striated sphincter.

Key words: intramural striated sphincter, nitric oxide synthase (NOS), urethra, rats