《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (06): 533-534.

• 基础研究 • 上一篇    下一篇

p75基因与2型糖尿病大鼠脑细胞凋亡相关性研究

任凤云; 宗灿华; 吕秀芳; 郭新民   

  1. 牡丹江医学院生物化学与分子生物学教研室,黑龙江牡丹江市 157011
  • 收稿日期:2007-12-10 出版日期:2008-06-01 发布日期:2008-06-01

Relationship between Expression of p75 Gene and Apoptosis of Cerebrum Cells in Type 2 Diabetic Rats

REN Feng-yun, ZONG Can-hua, LV Xiu-fang, et al   

  1. The Department of Biochemistry, Mudanjiang Medical College, Mudanjiang 157011, Heilonjiang, China
  • Received:2007-12-10 Published:2008-06-01 Online:2008-06-01

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被引次数

2

摘要： 目的探讨2型糖尿病大鼠p75基因表达与脑细胞凋亡的关系。方法选取2型糖尿病大鼠及正常对照组大鼠各10只,采用RT-PCR法检测脑组织p75基因mRNA表达,琼脂糖凝胶电泳检测细胞凋亡。结果2型糖尿病大鼠P75基因mRNA表达上调,且脑组织细胞凋亡明显增加。结论2型糖尿病大鼠脑组织细胞凋亡增加,与p75基因表达呈正相关。

关键词: 2型糖尿病大鼠, p75基因, 细胞凋亡

Abstract: Objective To study the relationship between expression of p75 gene and apoptosis of cerebrum cells in type 2 diabetic rats.Methods Ten type 2 diabetic rats and ten natural rats were used to test the expression of brain p75 mRNA by RT-PCR technique, and the apoptosis of cerebrum cells was measured by agarose gel electrophoresis (AGA).Results The expression of brain p75 mRNA and apoptosis of cerebrum cells in type 2 diabetic rats were higher than that of the natural group.Conclusion The apoptosis of cerebrum cells in type 2 diabetic rats increases, and it is positively correlated with the expression of brain p75 gene mRNA.

Key words: type 2 diabetic rat, p75 gene, apoptosis