《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (12): 1136-1139.

• 论文 • 上一篇    下一篇

经颅电刺激加康复训练对脑梗死大鼠功能恢复和生长相关蛋白-43的影响

杨丽霞1a，陈正红2，刘芳1b   

  1. 1. 贵阳医学院附属医院，a: 心理科；b: 神经科，贵州贵阳市 550004；2.贵州省交通医院综合病房，贵州贵阳市 550003。
  • 收稿日期:2010-08-31 修回日期:1900-01-01 出版日期:2010-12-25 发布日期:2010-12-25

Transcranial Electrical Stimulation and Rehabilitation Training on Functional Recovery and Growth Associated Protein-43 Expression in Rats after Focal Ischemic Infarction

YANG Li-xia, CHEN Zheng-hong, LIU Fang   

  1. Department of Psychology, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, China
  • Received:2010-08-31 Revised:1900-01-01 Published:2010-12-25 Online:2010-12-25

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摘要： 目的研究经颅电刺激加康复训练能否促进大鼠局部脑缺血再灌后的功能恢复，并从神经可塑性角度探讨其机理。方法线栓法制备SD大鼠短暂性大脑中动脉缺血再灌模型(MCAO)，术后随机分为电刺激加康复组、康复组和对照组。电刺激加康复组给予经颅电刺激和滚笼、平衡木训练，康复组仅给予滚笼、平衡木训练。分别于术后3、7、14和28 d用前肢放置试验(FPT)进行功能评分，以及用免疫组化法检测生长相关蛋白-43(GAP-43)在梗死灶周围的免疫活性强度。结果电刺激加康复组FPT评分在第7、14、28天优于康复组和对照组(P<0.01～0.05)；电刺激加康复组皮层缺血周边区GAP-43表达水平在第3、7和14天高于康复组和对照组(P<0.01～0.05)。结论经颅电刺激加康复训练能促进大鼠MCAO后肢体功能恢复，机制可能与上调缺血灶周边GAP-43的表达有关，而且作用优于单独的康复组。

关键词: 脑梗死, 经颅电刺激, 康复训练, 生长相关蛋白-43, 大鼠

Abstract: ObjectiveTo assess how transcranial electrical stimulation (TES) concurrent with rehabilitation training influences brain plasticity and behavioral functional performance in rats following a focal ischemic infarction.MethodsAfter an acute focal ischemic infarction by transient occlusion of right middle cerebral artery (MCAO), the electric stimulation concurrent with rehabilitation group was given TES, balancing and rotating and walking exercise everyday; the rehabilitation group was given only balancing and rotating and walking exercise everyday; the control group received no treatment. Growth associated protein 43(GAP-43) was examined by immunohistochemical techniques, and density of reaction product and forelimb placing test (FPT) were measured on the 3rd, 7th, 14th and 28th day after infarction respectively.ResultsThe electrical stimulation concurrent with rehabilitation group provided marked improvement in FPT on the 7th, 14th and 28th day compared with the rehabilitation group and the control group (P<0.01～0.05). The GAP-43 demonstrated statistically significant increase on the 3rd, 7th, and 14th day in the peri-infarct region in the electrical stimulation concurrent with rehabilitation group compared with other two groups(P<<0.01～0.05).ConclusionThe efficacy of transcranial electrical stimulation combining with rehabilitation training can improve functional outcome and neuronal plasticity following ischemic cerebral damage. The mechanism may be partly due to the upregulation of the expression of GAP-43 in the peri-infarct region. Meanwhile, the efficacy is superior to rehabilitation training only.

Key words: cerebral ischemic infarction, transcranial electrical stimulation, rehabilitation training, growth associated protein-43, rats