《中国康复理论与实践》

《中国康复理论与实践》 ›› 2010, Vol. 16 ›› Issue (9): 835-836.

• 论文 • 上一篇    下一篇

葛根素抑制脂多糖诱导RAW264.7巨噬细胞表达诱导型一氧化氮合酶

俞建1，胡文志1，季玲2，孙烈1   

  1. 1.南京医科大学第二附属医院心内科,江苏南京市 210011;2.南京医科大学第一附属医院心内科,江苏南京市 210029。
  • 收稿日期:2010-04-02 修回日期:2010-08-05 出版日期:2010-09-25 发布日期:2010-09-25

Puerarin Inhibits Inductible Nitric Oxide Synthase expression in Lipopolysaccharide -induced RAW264.7 Macrophage Cells

YU Jian, HU Wen-zhi, JI Ling, et al   

  1. Department of Cardiology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing 210011, Jiangsu, China
  • Received:2010-04-02 Revised:2010-08-05 Published:2010-09-25 Online:2010-09-25

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被引次数

5

摘要： 目的观察葛根素对脂多糖（LPS）诱导小鼠RAW264.7巨噬细胞表达诱导型一氧化氮合酶（iNOS）的影响。方法用脂多糖刺激小鼠巨噬细胞RAW264.7，与不同浓度的葛根素进行培养,采用逆转录多聚酶链式反应（RT-PCR）和免疫蛋白印迹法测定（Western blotting）方法分别检测RAW264.7巨噬细胞iNOS mRNA和蛋白质表达。结果随着葛根素的浓度的增加，RAW264.7细胞iNOS的mRNA及其蛋白质表达逐渐减少(P<0.01)。结论葛根素可以通过调节RAW264.7细胞表达iNOS发挥抗炎的作用。

关键词: 葛根素, 巨噬细胞, 脂多糖, 诱导型一氧化氮合酶

Abstract: ObjectiveTo investigate the effect of Puerarin on expression of inductible nitric oxide synthase (iNOS) in RAW264.7 macrophage cells induced by lipopolysaccharide （LPS）. MethodsThe protein and mRNA levels of lipopolysaccharide (LPS)-induced iNOS was determined in RAW264.7 macrophage cells using Western blotting and RT-PCR respectively. ResultsPuerarin inhibited expression of iNOS concentration dependently (P<0.01). ConclusionPuerarin may reduce iNOS level, result in prevention of inflammation.

Key words: Puerarin, macrophage cells, lipopolysaccharide, inductible nitric oxide synthase