《中国康复理论与实践》

《中国康复理论与实践》 ›› 2013, Vol. 19 ›› Issue (4): 318-323.

• 基础研究 • 上一篇    下一篇

生物活性材料支架诱导成年大鼠神经再生修复脑损伤的研究

陈兰1，孙敏1，张皑峰2，杨朝阳1，李晓光1   

  1. 1.首都医科大学神经生物学系，北京市 100069；2.北京友谊医院口腔科，北京市 100050
  • 收稿日期:2013-01-31 修回日期:2013-03-05 出版日期:2013-04-25 发布日期:2013-04-25
  • 通讯作者: 李晓光

Repair of Injured Adult Rat Brain by Inducing Neural Regeneration with Bioactive Material Scaffolds

CHEN Lan, SUN Min,ZHANG Ai-feng, et al   

  1. Department of Neurobiology, Capital Medical University, Beijing 100069, China
  • Received:2013-01-31 Revised:2013-03-05 Published:2013-04-25 Online:2013-04-25

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被引次数

8

摘要： 目的　应用生物活性材料支架诱导神经再生修复成年大鼠创伤性脑损伤。方法　52只成年雄性Wistar大鼠分为假手术组、单纯损伤组、单纯壳聚糖组和生物活性材料支架组，分别于术后3d、7d、2周、4周、2个月和3个月通过组织化学、免疫组织化学和神经示踪方法检测神经再生和损伤区新生神经元的来源。结果　应用生物活性材料支架后，海马齿状回(DG)和室管膜下区(SVZ)中BrdU+/Nestin+和BrdU+/Dcx+细胞较单纯壳聚糖组和单纯损伤组明显增加。损伤区内BrdU+/Nestin+、BrdU+/Dcx+及BrdU+/MAP2+神经细胞较单纯壳聚糖组和单纯损伤组明显增加。DiI标记SVZ细胞后，损伤区中见DiI+/NeuN+神经元。结论　生物活性材料支架激活DG和SVZ的神经干细胞，SVZ中的神经干细胞迁移到损伤区，并分化为成熟神经元。

关键词: 脑损伤, 生物活性材料支架, 神经干细胞, 神经再生, 迁移, 分化, 大鼠

Abstract: To repair the brain of adult rats with traumatic brain injury by inducing neural regeneration with biological scaffolds. Methods 52 adult male Wistar rats were divided into control group, lesion group, blank chitosan carriers group and bioactive material scaffolds group. The neural regeneration and the origin of the newborn neurons in the injury area were detected through histochemistry, immumochemistry and neural tracing on the 3rd day, 7th day, 2nd week, 4th week, 2nd month and 3rd month after operation. Results After application of bioactive material scaffolds, the BrdU+/Nestin+ and BrdU+/Dcx+ cells in gyrus dentatus (DG) of hippocampus and subventricular zone (SVZ) were significantly more than those of the other groups. The BrdU+/Nestin+, BrdU+/Dcx+ and BrdU+/MAP2+ neural cells in injury area of the bioactive material scaffolds group were significantly more than those of the other groups. After application of DiI to label the SVZ cells, DiI+/NeuN+ neurons were observed in the injury areas. Conclusion Bioactive material scaffolds can activate the neural stem cells in SVZ and DG. The neural stem cells in SVZ can migrate to the injury area and then differentiate into mature neurons.

Key words: brain injury, bioactive material scaffolds, neural stem cells, neural regeneration, migrate, differentiation, rats