《中国康复理论与实践》

《中国康复理论与实践》 ›› 2014, Vol. 20 ›› Issue (11): 1022-1027.

• 础基研究 • 上一篇    下一篇

整合素连接激酶对脐带间充质干细胞向神经干细胞样细胞分化的作用

周剑云，孙炜，张新，王建军，曹泽   

  1. 1.首都医科大学康复医学院，北京市100068；2.中国康复研究中心北京博爱医院外科，北京市100068。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-11-25 发布日期:2014-11-25

Effect of Integrin-linked Kinase on Derivation of Neural Stem-like Cells from Mesenchymal Stem Cells

ZHOU Jian-yun, SUN Wei,ZHANG Xin, WANG Jian-jun, CAO Ze   

  1. Capital Medical University School of Rehabilitation Medicine, Department of Surgery, Beijing Bo'ai Hospital, China Rehabilitation Research Centre, Beijing 100068, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-11-25 Online:2014-11-25

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摘要： 目的研究整合素连接激酶(ILK)对脐带间充质干细胞向神经干细胞样细胞分化的影响。方法分离人脐带间充质干细胞，用表达对照绿色荧光蛋白(GFP)和GFP融合野生型ILK、突变型ILK的质粒转染后，用含碱性成纤维细胞生长因子、表皮生长因子、B27 的无血清培养基培养。检测间充质干细胞的增殖速度及向神经干细胞样细胞分化的效率。结果转染ILK 的间充质干细胞中，ILK稳定表达，增殖速度加快，神经干细胞样细胞分化的效率提高；野生型ILK优于突变型ILK。结论ILK促进间充质干细胞向神经干细胞样细胞分化。其作用机制与激酶活性有关。

关键词: 整合素连接激酶, 间充质干细胞, 增殖, 神经干细胞样细胞

Abstract: Objective To investigate the effect of integrin-linked kinase (ILK) on inducing mesenchymal stem cells (MSCs) to neuron stem-like cell in vitro. Methods MSCs isolated from umbilical cord were transfected with plasmid DNA encoding green fluorescent protein (GFP) and GFP-fusion ILK, wild and mutant, respectively; and cultured in serum-free media with basic fibroblast growth factor, epidermal growth factor and B27. It was assessed with the proliferative activity and the efficiency of differentiation into neural stem-like cell. Results MSCs transfected with GFP-fusion wild ILK expressed ILK and kinase-dead mutant ILK stably. The activity of proliferation increased and more of them differentiated into neural stem-like cell, which express CD133 and Nestin validated by PCR and Western blotting, and more of wild than of mutant. Conclusion Integrin-linked kinase may play a positive role in MSC differentiation into neural stem-like cell, which may associate with the activity of kinase.

Key words: integrin-linked kinase, mesenchymal stem cells, proliferation, neural stem cell-like cell