《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (04): 402-405.

• 基础研究 • 上一篇    下一篇

番茄红素对大鼠脊髓损伤后白细胞介素-6表达及神经功能恢复的影响

李佳励 1，唐丽娟 2，李晓 2，曹钰 1，杨拯 2
   

  1. 1. 四川大学华西医院急诊科，四川成都市 610041；2.成都医学院基础医学实验教学中心, 四川成都市 610500。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-25 发布日期:2015-04-25

Effect of Lycopene on Expression of Interleukin-6 and Recovery of Neurological Function in Rats after Spinal Cord Injury

LI Jia-li1, TANG Li-juan2, LI Xiao1, CAO Yu1, YANG Zheng2
   

  1. 1. Department of Emergency Treatment, the West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China; 2. School of Basic Medical Science, Chengdu Medical College, Chengdu, Sichuan 610500, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-25 Online:2015-04-25

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被引次数

11

摘要： 目的 探讨番茄红素对大鼠脊髓损伤后白细胞介素-6 (IL-6)表达的影响及对神经功能恢复的作用。方法 健康成年Sprague-Dawley大鼠 36只，采用 Allen法(10 g×25 mm)在 T9造成大鼠脊髓损伤模型，并随机分为对照组(A组)、甲泼尼龙(MP)治疗组(B组)和番茄红素治疗组(C组)，每组12只。造模成功30 min后，A组不给予治疗，B组腹腔注射MP 30 mg/kg，C组灌胃番茄红素 20 mg/kg。于术后 1 d、3 d、7 d分别对各组进行斜板试验。损伤段脊髓切片行免疫组织化学染色检测 IL-6的表达。结果 术后1 d和 7 d，C组斜板试验评分均高于 A组(P<0.05)；术后 1 d、3 d和 7 d，B组斜板试验评分均高于 A组(P<0.05)。术后 1 d、3 d和 7d，B组和 C组 IL-6表达均显著低于 A组(P<0.001)。结论 番茄红素可以通过抑制 IL-6的表达，减轻急性脊髓损伤后的炎症反应，并促进大鼠神经和运动功能恢复。

关键词: 脊髓损伤, 番茄红素, 白细胞介素-6, 炎症, 大鼠

Abstract: Objective To explore the effects of lycopene on interleukin-6 (IL-6) expression and motor function after spinal cord injury in rats. Methods 36 healthy adult Sprague-Dawley rats were randomly divided into control group (A), methylprednisolone sodium succinate (MP) treatment group (B) and lycopene treatment group (C) with 12 rats in each group, and spinal cord injury model at T9 was established with modified Allen's technique (10 g×25 mm). 30 minutes after modeling, group A received no treatment, group B was injected MP 30 mg/ kg, group C was given lycopene 20 mg/kg. They were tested with inclined plate test 1 day, 3 days and 7 days after injury. The expression of IL-6 was examined with immunohistochemistry, then. Results The inclined plate test scores were higher in the group C than in the group A 1 day and 7 days after injury (P<0.05), and in the group B than in the group A 1 day, 3 days and 7 days after injury (P<0.05). The expression of IL-6 was significantly lower in the groups B and C than in the group A 1 day, 3 days, 7 days after injury (P<0.001). Conclusion Lycopene can inhibit the expression of IL-6 in acute spinal cord injury to reduce the inflammation and facilitate the recovery of nerve and motor function.

Key words: spinal cord injury, lycopene, interleukin-6, inflammation, rats