《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (04): 417-421.

• 基础研究 • 上一篇    下一篇

夹脊电针和神经松动术对兔坐骨神经损伤后轴突再生和血清神经营养因子的影响

王艳 1，王茜 2，陈国平 1，范红石 2
   

  1. 1.黑龙江中医药大学附属第二医院，黑龙江哈尔滨市 150001；2.黑龙江中医药大学，黑龙江哈尔滨市 150040。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-25 发布日期:2015-04-25

Effects of Jiaji Electroacupuncture and Neurodynamic Mobilization Technique on Axon Regeneration and Content of Neurotrophic Factors in Rabbits with Sciatic Nerve Injury

WANG Yan1, WANG Qian2, CHEN Guo-ping1, FAN Hong-shi1
   

  1. 1. The Second Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang 150001, China; 2. Heilongjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang 150040, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-25 Online:2015-04-25

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摘要： 目的 观察夹脊电针和神经松动术对兔坐骨神经损伤后轴突再生和血清脑源性神经营养因子(BDNF)、睫状神经营养 因子(CNTF)含量的影响。方法 30只成年雄性兔分为模型组(n=6)、假手术组(n=6)、神经松动术组(n=6)、夹脊电针组(n=6)、夹脊 电针结合神经松动术组(n=6)。钳夹法复制坐骨神经损伤模型。模型组、假手术组不做任何干预，神经松动术组行神经松动术治 疗，夹脊电针组进行夹脊电针治疗，夹电针结合神经松动术组进行夹脊电针和神经松动术治疗。治疗4周后，HE染色观察轴突生 长情况，ELISA法检测血清BDNF、CNTF含量。结果 神经松动术组、夹脊电针组、夹脊电针结合神经松动术组轴突生长情况均 优于模型组，夹脊电针结合神经松动术组优于神经松动术组和夹脊电针组；神经松动术组、夹脊电针组、夹脊电针结合神经松动 术组血清 BDNF、CNTF含量均高于模型组(P<0.05)，夹脊电针结合神经松动术组优于神经松动术组和夹脊电针组(P<0.05)。结论 神经松动术、夹脊电针均可促进兔损伤坐骨神经的轴突再生，可能与提高血清中CNTF、BDNF水平有关；两者结合效果更佳。

关键词: 周围神经损伤, 坐骨神经, 夹脊电针, 神经松动术, 脑源性神经营养因子, 睫状神经营养因子, 轴突再生, 兔

Abstract: Objective To observe the effects of Jiaji electroacupuncture and neurodynamic mobilization technique on axon regeneration and content of brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) in rabbits with sciatic nerve injury. Methods 30 male adult rabbits were assigned to sham (A, n=6), model (B, n=6), neurodynamic mobilization (C, n=6), Jiaji electroacupuncture (D, n=6), and Jiaji electroacupuncture combine with neurodynamic mobilization (E, n=6) groups. The group C was treated with neurodynamic mobilization, the group D with Jiaji electroacupuncture, and the group D with both 3 days after modeling of clamping at sciatic nerve, while the groups A and B with no treatment. The axon regeneration was observed with HE staining, and the content of BDNF and CNTF in serum was measured with ELISA 4 weeks after treatment. Results The axons regeneration was observed better in the groups C, D and E than in the group B. The content of BDNF and CNTF was more in the groups C, D and E than in the group B (P<0.05). Conclusion Both Jiaji electroacupuncture and neurodynamic mobilization can improve axon regeneration with synergistic action, which may associate with the increase of BDNF and CNTF in serum.

Key words: peripheral never injury, sciatic nerve, Jiaji electroacupuncture, neurodynamic mobilization technique, brain-derived neurotrophic factor, ciliary neurotrophic factor, axon regeneration, rabbits