《中国康复理论与实践》

《中国康复理论与实践》 ›› 2015, Vol. 21 ›› Issue (09): 1020-1024.

• 基础研究 • 上一篇    下一篇

电针对脑缺血再灌注大鼠学习记忆能力及海马组织脑源性神经营养因子和神经营养素受体p75表达的影响

李晓洁 1，林如辉 2，陶静 1，赵从快 1，俞坤强 1，李春燕 1，彭洪卫 1，陈立典 3   

  1. 1.福建中医药大学康复医学院，福建福州市 350122；2.福建中医药大学中西医结合研究院，福建福州市 350122；3.福建中医药大学，福建福州市 350122。
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-09-25 发布日期:2015-09-25

Effects of Electroacupuncture on Learning and Memory and Expression of Brain-derived Neurotrophic Factor and p75 Neurotrophin Receptor in Rats with Cerebral Ischemia-reperfusion

LI Xiao-jie1, LIN Ru-hui2, TAO Jing1, ZHAO Cong-kuai1, YU Kun-qiang1, LI Chun-yan1, PENG Hong-wei1, CHEN Li-dian3   

  1. 1. Rehabilitation Medicine College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China; 2. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China; 3. Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-09-25 Online:2015-09-25

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摘要： 目的 探讨电针治疗脑卒中后认知障碍的可能机制。方法 45只 Sprague-Dawley雄性大鼠随机分为假手术组(n=15)、模型组(n=15)和电针组(n=15)。模型组、电针组用线栓法制备左侧局灶性脑缺血 1.5 h再灌注模型。电针组电针神庭、百会穴 7 d。采用 Morris水迷宫检测大鼠学习记忆能力； Longa评分法观察大鼠神经功能缺损情况；HE染色观察大鼠海马神经元形态结构变化；免疫印迹法检测大鼠缺血侧海马组织中脑源性神经营养因子(BDNF)、神经营养素受体p75 (p75NTR)蛋白表达。结果 与模型组相比，电针组逃避潜伏期缩短(P<0.05)，穿越平台次数显著增加(P<0.001)；神经功能缺损评分明显减少(P<0.01)；缺血侧海马区细胞损伤较轻，BDNF表达明显升高(P<0.01)，p75NTR蛋白表达明显降低(P<0.01)。结论 电针能够改善脑缺血再灌注大鼠学习记忆能力，其机制可能与上调大鼠海马组织中BDNF蛋白和下调p75NTR蛋白表达水平有关。

关键词: 脑缺血再灌注, 学习记忆, 电针, 脑源性神经营养因子, 神经营养素受体p75, 大鼠

Abstract: Objective To observe the effects of electroacupuncture on learning and memory, and discuss the therapeutic mechanism. Methods 45 Sprague-Dawley rats were randomly divided into sham group (n=15), model group (n=15) and electroacupuncture group (n= 15). The latter 2 groups were modeled with middle cerebral artery occlusion for 1.5 h and reperfusion. The rats of electroacupuncture group received electroacupuncture at Shenting (DU24) and Baihui (DU20) for 7 days. Learning and memory ability was tested with Morris water maze. Neurologic impairment was assessed with Longa's score. Their hippocampus were observed under HE staining and the level of brain-derived neurotrophic factor (BDNF) and p75 neurotrophin receptor (p75NTR) protein were determined with Western blotting. Results Compared with the model group, the latency of water maze decreased and the times crossing the platform increased in electroacupuncture group (P<0.05), while the Longa's score significantly reduced (P<0.05), and the lesion of nerve cells were alleviated, with the decrease of p75NTR and increase of BDNF in the ischemic hippocampus (P<0.01). Conclusion Electroacupuncture can improve the learning and memory of cerebral ischemia-reperfusion rats, which may relate with up-regulating BDNF and down-regulating p75NTR in hippocampus.

Key words: cerebral ischemia-reperfusion, learning and memory, electroacupuncture, brain-derived neurotrophic factor, p75 neurotrophin receptor, rats