《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

脉冲射频对慢性坐骨神经缩窄伤大鼠坐骨神经超微结构和胶质细胞源性神经营养因子表达的影响①

贾子普，任浩，李倩，王婧爱，罗芳   

  1. 首都医科大学附属北京天坛医院麻醉科，北京市100050。作者简介：贾子普(1990-)，男，汉族，河北徐水县人，硕士研究生，医师，主要研究方向：慢性疼痛的诊疗。
  • 出版日期:2016-01-25 发布日期:2016-05-23

Effects of Pulsed Radiofrequency on Ultrastructure and Glial Cell Line-derived Neurotrophic Factor Expression in Sciatic Nerve Chronic Constriction Injury Rats

JIA Zi-pu, REN Hao, LI Qian, WANG Jing-ai, LUO Fang   

  1. Department of Anesthesiology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
  • Published:2016-01-25 Online:2016-05-23

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摘要： 目的观察脉冲射频(PRF)对慢性坐骨神经损伤大鼠坐骨神经机械痛阈、超微结构及胶质细胞源性神经营养因子(GDNF)表达的影响。方法30 只Sprague-Dawley大鼠分为假手术假治疗组(SS 组, n=10)、造模后假治疗组(CS组, n=10)、造模后脉冲射频治疗组(CP 组, n=10)。CS组和CP组大鼠复制坐骨神经慢性缩窄伤(CCI)模型，SS组接受假手术。造模后14 d，CP组于坐骨神经干结扎处接受脉冲射频治疗3 min；SS 组和CS组仅暴露坐骨神经干放置电极不通电。造模前，造模后1 d、7 d、14 d ，治疗后1 d、7 d、14 d 测量各组机械缩足阈值(HWT)。治疗后14 d 取各组坐骨神经干结扎处，电镜观察超微结构，酶联免疫吸附法测定GDNF水平。结果造模后，CS、CP 组大鼠HWT较SS 组明显降低(P<0.01)；治疗14 d，CP 组HWT较CS 组明显升高(P<0.01)。电镜下可见CP组结扎侧坐骨神经损伤较CS组减轻，GDNF表达明显高于CS组和SS组(P<0.01)。结论PRF可能通过上调坐骨神经干中GDNF的表达，保护坐骨神经，缓解CCI所致的坐骨神经病理性疼痛。

关键词: 神经病理性疼痛, 坐骨神经, 脉冲射频, 慢性缩窄伤, 胶质细胞源性神经营养因子, 大鼠

Abstract: Objective To observe the hindpaw withdrawal threshold (HWT), and the ultrastructure and expression of glia cell line-derived neurotrophic factor (GDNF) in sciatic nerve (SN) in chronic constriction injury (CCI) rats after pulsed radiofrequency (PRF). Methods 30 Sprague-Dawley rats were divided into sham modeling-sham treating (SS) group, CCI-Sham treating (CS) group and CCI-PRF (CP) group. The right SNs of the rats in the CS and CP groups were ligated, and it was separated without ligation in the SS group. The CP group accepted PRF at the ligation 14 days after modeling, while the electrodes were placed without electricity in the SS and CS groups. Their HWT was measured before and 1, 7, 14 days after modeling, and 1, 7, 14 days after treatment. The right SN of ligation was observed under electron microscope 14 days after treatment, meanwhile, the GDNF expression was determined with enzyme-linked immunosorbent assay (ELISA). Results HWT was significantly shorter in the CS and CP groups than in the SS group after modeling, and it increased in the CP group 14 days after treatment compared with that of the CS group (P<0.01). The degeneration of SN significantly improved in the CP group compared with the CS group, while the expression of GDNF increased compared with that in the CS and SS groups (P<0.01). Conclusion PRF could relieve the CCI-induced neuropathic pain by upregulating the GDNF expression in the SN to prevent the SN from injury.

Key words: neuropathic pain, sciatic nerve, pulsed radiofrequence, chronic constriction injury, glial cell line-derived neurotrophic factor, rats