《中国康复理论与实践》

《中国康复理论与实践》

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热射病对大鼠空间学习记忆能力远期损害的研究①

张云1,2，万明胜3，董会1，吴士文2   

  1. 1.新乡医学院，河南新乡市453000；2.武警总医院，北京市100039；3.安徽医科大学，安徽合肥市230000。
  • 出版日期:2016-02-25 发布日期:2016-06-01

Long-term Impairment of Spatial Learning and Memory in Rats after Heat Stroke

ZHANG Yun1,2, WAN Ming-sheng3, DONG Hui1, WU Shi-wen2   

  1. 1. Xinxiang Medical University, Xinxiang, Henan 453000, China; 2.The General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China; 3. Anhui Medical University, Hefei, Anhui 230000, China
  • Published:2016-02-25 Online:2016-06-01

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摘要： 目的比较热射病大鼠不同阶段的空间学习记忆能力，探讨其远期影响。方法Sprague-Dawley 大鼠42 只，建立热射病大鼠模型，将造模成功后的大鼠随机分为热射病7 d 组(HS7，n=21)和热射病21 d 组(HS21，n=21)；另取18 只大鼠麻醉后行股动脉插管作为手术对照组(Sham，n=18)。3 组分别于造模后7 d、21 d 行Morris 水迷宫实验5 d，记录逃避潜伏期，跨越平台的次数和目标象限停留时间。结果逃避潜伏期各个时间点HS7组均较Sham 组延长(P<0.05)；第4、5 天，HS21组较Sham 组延长(P<0.05)；第3、4、5 天，HS21组较HS7组缩短(P<0.05)。目标象限停留时间HS7组较Sham 组缩短(P<0.05)；HS21组较HS7组延长(P<0.05)。穿越平台次数HS7组和HS21组较Sham 组减少(P<0.05)；HS21组与HS7组无显著性差异(P>0.05)。结论热射病大鼠空间学习记忆障碍在发病7 d 后最严重，并可遗留远期损害。

关键词: 热射病, 空间学习记忆, Morris 水迷宫, 远期损害, 大鼠

Abstract: Objective To compare the spatial learning and memory function of heat stroke rats in different periods, to explore the long- term impairment. Methods 42 Sprague-Dawley rats were randomly divided into heat stroke 7 days group (HS7, n=21), heat stroke 21 days group (HS21, n=21), and another 18 rats were performed femoral artery intubation as surgery control group (sham, n=18). They were tested with Morris water maze 7 days and 21 days after modeling respectively for 5 days. The escaping latency, the frequency of crossing the platform area and the duration in the target quadrant were recorded. Results Compared with the sham group, the escaping latency prolonged in HS7 group in all the time (P<0.05), and 4th and 5th days in HS21 group (P<0.05). Compared with HS7 group, the escaping latency shortened in HS21 group on the 3rd, 4th and 5th days (P<0.05). The duration in the target quadrant shortened in HS7 group compared with the sham group (P<0.05), and prolonged in HS21 group compared with HS7 group (P<0.05). The frequency of crossing the platform decreased both in HS7 group and HS21 group compared with the sham group (P<0.05), and no difference was found between the later 2 groups (P> 0.05). Conclusion The impairment of spatial learning and memory is the most seriously 7 days after heat stroke in rats, and it may remain for long time.

Key words: heat stroke, spatial learning and memory, Morris water maze, long-term impairment, rats