《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

脊髓损伤后水通道蛋白-4在脊髓白质中的表达①

向虹雨，李胜男，曾茜，张晓，梁楠   

  1. 成都医学院基础医学实验教学中心，四川成都市 610500。
  • 出版日期:2016-04-25 发布日期:2016-06-01

Expression of Aquaporin-4 in White Matter of Spinal Cord in Rats after Spinal Cord Contusion

XIANG Hong-yu, LI Sheng-nan, ZENG Xi, ZHANG Xiao, LIANG Nan   

  1. Center for Experimental Technology for Preclinical Medicine, Chengdu Medical College, Chengdu 610500, Sichuan, China
  • Published:2016-04-25 Online:2016-06-01

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摘要： 目的 探讨脊髓挫伤(SCC)后水通道蛋白-4(AQP-4)在脊髓白质中的表达变化。方法 成年健康雌性Sprague-Dawley大鼠 88只，随机分为假手术组(Sham)和 SCC组。Allen打击法建立 SCC模型，BBB评分评价大鼠运动功能变化情况；免疫组织化学染色检测AQP-4在脊髓白质中不同时间点的定位情况；Q-PCR检测AQP-4 mRNA含量变化。结果 SCC组BBB评分明显降低，损伤后7 d和14 d明显升高(t>5.061, P<0.01)。Q-PCR结果显示，术后1 d和3 d AQP-4 mRNA表达显著下降(t>50.44, P<0.001)，损伤后5 d，AQP-4 mRNA表达水平较 3 d明显上升(t=-3.968, P=0.001)，直至 28 d仍明显高于 3 d(t=-4.227, P=0.001)。免疫组织化学染色结果显示，AQP-4主要定位于脊髓白质的血管内皮细胞和神经胶质细胞的突起。结论 SCC后，AQP-4可能在脊髓损伤后不同时期发挥不同的病理生理作用。

关键词: 脊髓挫伤, 水肿, 水通道蛋白-4, 大鼠

Abstract: Objective To explore the expression of aquaporin (AQP)-4 in white matter of spinal cord after spinal cord contusion (SCC). Methods 88 adult Sprague-Dawley rats were assigned randomly to sham operation group and SCC group. The model was established by Allen's method. BBB sore was used to assess the motor function of rats. The relative expression of AQP-4 mRNA was determined by Q-PCR technique. The localization of AQP-4 was observed by immunohistochemistry. Results BBB score showed motor dysfunction in SCC group, and it increased 7 and 14 days after SCC (t>5.061, P<0.001). The level of AQP-4 mRNA decreased on the 1st and 3rd days (t>50.44, P< 0.001), and increased on the 5th day (t=-3.968, P=0.001), and lasted until the 28th day (t=-4.227, P=0.001) compared with that on the 3rd day. The immunohistochemistry showed AQP-4 was located on the process of glial cell and vascular endothelial cells in white matter of spinal cord. Conclusion AQP-4 may play various roles at different stages in SCC.

Key words: spinal cord contusion, edema, aquaporin-4, rats