冻结肩患者肩关节致纤维化细胞因子和炎症细胞因子的表达

doi:10.3969/j.issn.1006-9771.2017.01.023

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (1): 97-100.doi: 10.3969/j.issn.1006-9771.2017.01.023

冻结肩患者肩关节致纤维化细胞因子和炎症细胞因子的表达

闵红巍, 刘克敏, 曲铁兵, 亓攀, 顾蕊

1.首都医科大学康复医学院,北京市 100068;
2.中国康复研究中心北京博爱医院骨科,北京市 100068

收稿日期:2016-09-22 出版日期:2017-01-20 发布日期:2017-02-17
通讯作者: 刘克敏。E-mail: keminlqliu@sina.com。
作者简介:闵红巍(1971-),男,汉族,黑龙江哈尔滨市人,博士,副主任医师,主要研究方向：骨关节康复。
基金资助:
北京丰台区卫生系统科学研究项目(No.2014-丰台); 中央级公益性科研院所基本科研业务费专项资金项目(No.2015CZ-13)

Expression of Fibrogenic Cytokine and Inflammatory Cytokine in Frozen Shoulder

MIN Hong-wei, LIU Ke-min, QU Tie-bing, QI Pan, GU Rui

1. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China;
2. Department of Orthopedics and Rehabilitation, Beijing Bo'ai Hospital, China Rehabilitation Research Center, Beijing 100068, China

Received:2016-09-22 Published:2017-01-20 Online:2017-02-17
Contact: LIU Ke-min. E-mail: keminlqliu@sina.com

摘要/Abstract

摘要： 目的探讨致纤维化细胞因子和炎症细胞因子在冻结肩发生中的可能作用。方法 2014年9月至2016年4月,20例冻结肩患者接受肩关节镜手术,其中原发性冻结肩10例(A组),继发性冻结肩10例(B组)。以同期接受肩关节镜手术的10例非冻结肩患者作为对照(C组)。qPCR检测肩关节滑膜组织中基质金属蛋白酶1(MMP1)、MMP3、肿瘤坏死因子-α (TNF-α)、白细胞介素-1 (IL-1)、IL-6、IL-8、粒细胞巨噬细胞集落刺激因子(GM-CSF)和巨噬细胞集落刺激因子(M-CSF) mRNA表达水平。结果 A组和B组MMP1和MMP3 mRNA表达高于C组(P<0.05),TNF-α、IL-1、IL-6、IL-8、GM-CSF和M-CSF mRNA显著高于C组(P<0.001),A组和B组间各因子mRNA表达无显著性差异(P>0.05)。结论冻结肩的发生可能与患者关节滑膜组织中致纤维化细胞因子和炎症细胞因子的高表达有关。

关键词: 冻结肩, 滑膜, 纤维化, 炎症, 细胞因子

Abstract: Objective To explore the role of fibrogenic cytokines and inflammatory cytokines in the pathogenesis of frozen shoulder. Methods From September, 2014 to April, 2016, 20 patients with frozen shoulder accepted arthroscopic surgery were included, ten of them were diagnosed as primary frozen shoulder (group A), the other ten were secondary frozen shoulder (group B). Other ten patients undergoing shoulder arthroscopy for instability (4 cases), rotator cuff injury (3 cases) and subacromial impingement (3 cases) were as the controls (group C). The mRNA levels of matrix metalloproteinase (MMP) 1, MMP3, tumour necrosis factor-α (TNF-α), interleukin (IL)-1, IL-6, IL-8, granulocyte-macrophage colony stimulating factor (GM-CSF) and M-CSF in synovium were analyzed with quantitative polymerase chain reaction. Results The expression of mRNA of MMP1, MMP3, TNF-α, IL-1, IL-6, IL-8, GM-CSF and M-CSF were more in group A and group B than in group C (P<0.05). There was no significant difference between group A and group B (P>0.05). Conclusion The fibrogenic cytokines and inflammatory cytokines may play a role in the pathogenesis of frozen shoulder.

Key words: frozen shoulder, synovium, fibration, inflammation, cytokine

中图分类号:

R684

闵红巍, 刘克敏, 曲铁兵, 亓攀, 顾蕊. 冻结肩患者肩关节致纤维化细胞因子和炎症细胞因子的表达[J]. 《中国康复理论与实践》, 2017, 23(1): 97-100.

MIN Hong-wei, LIU Ke-min, QU Tie-bing, QI Pan, GU Rui. Expression of Fibrogenic Cytokine and Inflammatory Cytokine in Frozen Shoulder[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(1): 97-100.

参考文献

[1] Sherian MA, Hannafin JA. Upper extremity. Emphasis of frozen shoulder [J]. Orthop Clin North Am, 2006, 37(4): 531-539.
[2] Tauro JC, Paulson M. Shoulder stiffness [J]. Arthroscopy, 2008, 24(8): 949-955.
[3] Duplay ES. De la Periarthrite scapulohumerale [J]. Arch Gen Med, 1872, 20: 513-542.
[4] Codman EA. Tendinitis of short rotators [M]// Codman EA. The Shoulder: Rupture of the Supraspinatus Tendon and Other Lesions in or about the Subacromial Bursa. Boston: Thomas Todd, 1934.
[5] Neviaser JS. Adhesive capsulitis of the shoulder–A study of the pathological findings in periarthritis of the shoulder [J]. J Bone Joint Surg, 1945, 27(2): 211-222.
[6] Kabbabe B, Ramkumar S, Richardson M. Cytogenetic analysis of the pathology of frozen shoulder [J]. Int J Shoulder Surg, 2010, 4(3): 75-78.
[7] Mullett H, Byrne D, Colville J. Adhesive capsulitis: human fibroblast response to shoulder joint aspirate from patients with stage II disease [J]. J Shoulder Elbow Surg, 2007, 16(3): 290-294.
[8] Rangan A, Goodchild L, Gibson J, et al. Frozen shoulder [J]. Shoulder Elbow, 2015, 7(4): 299-307.
[9] Okamura K, Ozaki J. Bone mineral density of the shoulder joint in frozen shoulder [J]. Arch Orthop Trauma Surg, 1999, 119(7-8): 363-367.
[10] Müller LP, Müller LA, Happ J, et al. Frozen shoulder: a sympathetic dystrophy? [J]. Arch Orthop Trauma Surg, 2000, 120(1-2): 84-87.
[11] Smith SP, Devaraj VS, Bunker TD. The association between frozen shoulder and Dupuytren's disease [J]. J Shoulder Elbow Surg, 2001, 10(2): 149-151.
[12] Bunker TD, Anthony PP. The pathology of frozen shoulder. A Dupuytren-like disease [J]. J Bone Joint Surg Br, 1995, 77(5): 677-683.
[13] Nho SJ, Delos D, Yadav H, et al. Biomechanical and biologic augmentation for the treatmentof massive rotator cuff tears [J]. Am J Sports Med, 2010, 38(3): 619-629.
[14] Longo UG, Rizzello G, Berton A, et al. Biological strategies to enhance rotator cuff healing [J]. Curr Stem Cell Res Ther, 2013, 8(6): 464-470.
[15] Blaine TA, Kim YS, Voloshin I, et al. The molecular pathophysiology of subacromial bursitis in rotator cuff disease [J]. J Shoulder Elbow Surg, 2005, 14(1 Suppl): 84S-89S.
[16] Voloshin I, Gelinas J, Maloney MD, et al. Proinflammatory cytokines and metalloproteases are expressed in the subacromial bursa in patients with rotator cuff disease [J]. Arthroscopy, 2005, 21(9): 1076.e1-1076.e9.
[17] Crofford LJ. COX-1 and COX-2 tissue expression: implications and predictions [J]. J Rheumatol Suppl, 1997, 49(7): 15-19.
[18] Zimowska M, Kasprzycka P, Bocian K, et al. Inflammatory response during slow- and fast-twitch muscle regeneration [J]. Muscle Nerve, 2016-07-11. [Epub ahead of print]
[19] Lho YM, Ha E, Cho CH, et al. Inflammatory cytokines are overexpressed in the subacromial bursa of frozen shoulder [J]. J Shoulder Elbow Surg, 2013, 22(5): 666-672.
[20] Fossati G, Mazzucchelli I, Gritti D, et al. In vitro effects of GM-CSF on mature peripheral blood neutrophils [J]. Int J Mol Med, 1998, 1(6): 943-951.
[21] Ji XH, Sun LH, Qin JC, et al. Effects of rhM-CSF expressed in silkworm on cytokine productions and membrane molecule expressions of human monocytes [J]. Acta Pharmacol Sin, 2000, 21(9): 797-801.

冻结肩患者肩关节致纤维化细胞因子和炎症细胞因子的表达

Expression of Fibrogenic Cytokine and Inflammatory Cytokine in Frozen Shoulder

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