《中国康复理论与实践》

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (2): 162-165.doi: 10.3969/j.issn.1006-9771.2017.02.009

• 基础研究 • 上一篇    下一篇

深部脑磁刺激术对慢性束缚应激大鼠习得性无助样绝望行为的疗效

刘博1, 2, 张攀1, 2, 李达1, 2, 杨予涛1, 2, 郑云峰3, 徐志卿1, 2   

  1. 1.首都医科大学神经生物学系,北京市 100069;
    2. 首都医科大学北京脑重大疾病研究院,北京市 100069;
    3. 北京澳丹斯生物科技有限公司,北京市 100044
  • 收稿日期:2016-09-23 修回日期:2016-12-05 出版日期:2017-02-05 发布日期:2017-03-06
  • 通讯作者: 徐志卿(1963-),男,浙江台州市人,博士,教授,主要研究方向：脑重大疾病中神经肽及受体的作用及其分子机制研究。E-mail: zhiqingxu@ccmu.edu.cn。
  • 作者简介:刘博(1990-),男,汉族,辽宁彰武县人,硕士研究生,主要研究方向：在慢性应激动物模型上探究深部脑磁刺激的抗抑郁抗绝望作用。
  • 基金资助:
    1.国家自然科学基金面上项目(No.81671345; No.31171032; No.31271154); 2.北京市自然科学基金重点项目(No.7091002); 3.北京市自然科学基金重点B类项目(No.KZ201610025026)

Effect of Deep-brain Magnetic Stimulation on Learned Helplessness Behavior in Rats with Chronic Restrained Stress

LIU Bo1, 2, ZHANG Pan1, 2, LI Da1, 2, YANG Yu-tao1, 2, ZHENG Yun-feng3, XU Zhi-qing1, 2   

  1. 1. Department of Neurobiology, Capital Medical University, Beijing 100069, China;
    2. Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China;
    3. Beijing Aldans Bio-tech Co., Ltd., Beijing 100044, China
  • Received:2016-09-23 Revised:2016-12-05 Published:2017-02-05 Online:2017-03-06
  • Contact: XU Zhi-qing. E-mail: zhiqingxu@ccmu.edu.cn

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摘要： 目的 探讨深部脑磁刺激术(DMS)对慢性束缚应激(CRS)大鼠模型习得性无助样绝望行为的治疗作用。方法 29只Sprague-Dawley大鼠随机分为对照组(n=8)和CRS组(n=21)。CRS组予以束缚应激,对照组不做任何应激处理。3周后,通过强迫游泳实验(FST)观察大鼠习得性无助样绝望行为,将具有绝望行为的CRS组大鼠随机分为假治疗组(n=6)、DMS组(n=8)和西酞普兰组(n=7),并接受相应治疗。1周后,观察各组在FST中的不动时间。结果 CRS组在FST中不动时间明显高于对照组(F=11.260, P=0.002)。治疗1周后,西酞普兰组FST中的不动时间无显著性差异(F=1.565, P=0.235);DMS组不动时间短于假治疗组(F=5.560, P=0.036),也短于治疗前(F=6.277, P=0.025)。结论 CRS可导致大鼠产生习得性无助样绝望行为,为期1周DMS治疗可改善该行为。

关键词: 慢性束缚应激, 习得性无助样绝望行为, 深部脑磁刺激, 绝望, 自杀, 抑郁, 大鼠

Abstract: Objective To explore the curative effect of deep-brain magnetic stimulation (DMS) on learned helplessness behavior in the chronic restrained stress (CRS) rat model. Methods Twenty-nine Sprague-Dawley rats were randomly divided into control group (n=8) and CRS group (n=21). CRS group was exerted chronic restrained stress, while the control group did not receive any stress, for three weeks. Then learned helplessness behavior was tested using Forced Swimming Test (FST) and the hopeless rats of the CRS group were divided randomly into sham group (n=6), DMS group (n=8) and citalopram group (n=7), that received corresponding treatment respectively. They were evaluated with FST again after one-week treatment. Results The immobile time in FST was longer in CRS group than in the control group after three-week stress (F=11.260, P=0.002). After one-week treatment, no significant improvement was found in the citalopram group (F=1.565, P=0.235), however, the immobile time in DMS group decreased (F=6.277, P=0.025), and was shorter than that in the sham group (F=5.560, P=0.036). Conclusion CRS could result in learned helplessness behavior, which could be alleviated with one-week DMS.

Key words: chronic restrained stress, learned helplessness behavior, deep-brain magnetic stimulation, hopelessness, suicide, depression, rats

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