运动或电针预处理对脑梗死大鼠神经功能缺损及层粘连蛋白表达的影响

doi:10.3969/j.issn.1006-9771.2017.03.008

《中国康复理论与实践》 ›› 2017, Vol. 23 ›› Issue (3): 288-291.doi: 10.3969/j.issn.1006-9771.2017.03.008

运动或电针预处理对脑梗死大鼠神经功能缺损及层粘连蛋白表达的影响

唐强¹, 阮野², 李宏玉², 叶涛², 吴孝军², 田源², 朱路文¹

1.黑龙江中医药大学附属第二医院,黑龙江哈尔滨市 150001;
2.黑龙江中医药大学,黑龙江哈尔滨市 150040。

收稿日期:2016-11-15 出版日期:2017-03-05 发布日期:2017-03-31
通讯作者: 朱路文(1983-),男,汉族,山东邹城市人,博士,副教授,主要研究方向：神经系统疾病中医康复基础与临床研究。E-mail: zhuluwen1983@126.com。
作者简介:唐强(1963-),男,汉族,四川大竹县人,博士,教授,主要研究方向：神经系统疾病中医康复基础与临床研究。
基金资助:
1.国家自然科学基金项目(No.81503666); 2.黑龙江中医药大学领军人才计划项目(No.2012RCL02); 3.黑龙江中医药大学研究生创新科研项目(No.yjscx2016037)

Effects of Exercise or Electroacupuncture Preconditioning on Neurological Deficits and Expression of Laminin in Rats with Cerebral Infarction

TANG Qiang¹, RUAN Ye², LI Hong-yu², YE Tao², WU Xiao-jun², TIAN Yuan², ZHU Lu-wen¹

1. Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China;
2. Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, China

Received:2016-11-15 Published:2017-03-05 Online:2017-03-31
Contact: Correspondence to ZHU Lu-wen. E-mail: zhuluwen1983@126.com

摘要/Abstract

摘要： 目的探讨运动预处理与电针预处理对局灶性脑梗死大鼠神经功能的影响及其可能机制。方法24只雄性Sprague-Dawley大鼠随机分为模型组(n=6)、假手术组(n=6)、运动预处理组(n=6)和电针预处理组(n=6)。模型组、假手术组不行任何处理;运动预处理组行跑台训练2周,电针预处理组行电针预处理2周。采用改良线栓法制作大鼠局灶性脑梗死模型,假手术组行相同血管结扎,不造成梗死。24 h后采用改良神经功能缺损评分(NSS)对大鼠进行评定,Western blotting检测脑缺血区层粘连蛋白的表达。结果假手术组未见神经功能缺损表现,预处理组NSS评分低于模型组(P<0.05),两预处理组间无显著性差异(P>0.05)。假手术组层粘连蛋白表达最高,模型组较预处理组表达降低(P<0.05),两预处理组间无显著性差异(P>0.05)。结论运动预处理与电针预处理均能降低局灶性脑梗死大鼠神经功能缺损程度,可能与抑制层粘连蛋白早期表达降低有关。

关键词: 局灶性脑梗死, 预处理, 运动, 电针, 神经功能, 层粘连蛋白, 大鼠

Abstract: ObjectiveTo investigate the effect of electroacupuncture or exercise preconditioning on neurological function after focal cerebral infarction in rats and the possible mechanism. MethodsA total of 24 male Sprague-Dawley rats were randomly divided into model group (n=6), sham group (n=6), exercise preconditioning group (n=6) and electroacupuncture preconditioning group (n=6). The model group and the sham group did not accept any treatment, while the exercise preconditioning group and the electroacupuncture preconditioning group accepted treadmill training and electroacupuncture for two weeks, respectively. Their middle cerebral arteries were occluded with modified Longa's approach, except the sham group that was ligated the same arteries but did not result in infarction. They were evaluated with Neurologic Severity Scores (NSS) 24 hours after modeling, and the laminin expression in the ischemic area was detected with Western blotting. ResultsThere was no neurological deficit in the sham group. The NSS was lower in both preconditioning groups than in the model group (P<0.05), but was not significant different between preconditioning groups (P>0.05). The expression of laminin was the most in the sham group, and was more in both preconditioning groups than in the model group (P<0.05), but was not significant different between preconditioning groups (P>0.05). ConclusionPreconditioning with exercise or electroacupuncture can both reduce the neurological deficits in rats after focal cerebral infarction, which may associate with the protection of laminin from inhibition in early stage.

Key words: focal cerebral infarction, preconditioning, exercise, electroacupuncture, neurological function, laminin, rats

中图分类号:

R743.32

唐强, 阮野, 李宏玉, 叶涛, 吴孝军, 田源, 朱路文. 运动或电针预处理对脑梗死大鼠神经功能缺损及层粘连蛋白表达的影响[J]. 《中国康复理论与实践》, 2017, 23(3): 288-291.

TANG Qiang, RUAN Ye, LI Hong-yu, YE Tao, WU Xiao-jun, TIAN Yuan, ZHU Lu-wen. Effects of Exercise or Electroacupuncture Preconditioning on Neurological Deficits and Expression of Laminin in Rats with Cerebral Infarction[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(3): 288-291.

参考文献

[1] Meschia JF, Bushnell C, Boden-Albala B, et al. Guidelines for the Primary Prevention of Stroke. A Statement for Healthcare Professionals from the American Heart Association/American Stroke Association [J]. Stroke, 2014, 45(12): 3754-3832.
[2] Wang W, Jiang B, Sun H, et al. Prevalence, incidence and mortality of stroke in China: results from a Nationwide Population-Based Survey of 480,687 Adults [J]. Circulation, 2017. [Epub ahead of print]. doi:10.1161/CIRCULATIONAHA.116.025250.
[3] Otsuka S, Sakakima H, Sumizono M, et al. The neuroprotective effects of preconditioning exercise on brain damage and neurotrophic factors after focal brain ischemia in rats [J]. Behav Brain Res, 2016, 303: 9-18.
[4] Feng R, Zhang F. The neuroprotective effect of electro-acupuncture against ischemic stroke in animal model: a review [J]. Afr J Tradit Complement Altern Med, 2014, 11(3): 25-29.
[5] 叶涛,朱路文,唐强. 电针预处理诱导脑缺血耐受的研究进展[J]. 针灸临床杂志, 2015, 31(2): 87-90.
[6] Krueger M, Bechmann I, Immig K, et al. Blood-brain barrier breakdown involves four distinct stages of vascular damage in various models of experimental focal cerebral ischemia [J]. J Cereb Blood Flow Metab, 2015, 35(2): 292-303.
[7] Kim SJ, Lee SR. Protective effect of melatonin against transient global cerebral ischemia-induced neuronal cell damage via inhibition of matrix metalloproteinase-9 [J]. Life Sci, 2014, 94(1): 8-16.
[8] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20(1): 84-91.
[9] Chen J, Li Y, Wang L, et al. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats [J]. Stroke, 2001, 32(4): 1005-1011.
[10] Ding YH, Ding Y, Li J, et al. Exercise pre-conditioning strengthens brain microvascular integrity in a rat stroke model [J]. Neurol Res, 2006, 28(2): 184-189.
[11] Kang KA, Seong H, Jin HB, et al. The effect of treadmill exercise on ischemic neuronal injury in the stroke animal model: potentiation of cerebral vascular integrity [J]. J Korean Acad Nurs, 2011, 41(2): 197-203.
[12] Zhang Q, Zhang L, Yang X, et al. The effects of exercise preconditioning on cerebral blood flow change and endothelin-1 expression after cerebral ischemia in rats [J]. J Stroke Cerebrovasc Dis, 2014, 23(6): 1696-1702.
[13] Curry A, Guo M, Patel R, et al. Exercise pre-conditioning reduces brain inflammation in stroke via tumor necrosis factor-α, extracellular signal-regulated kinase 1/2 and matrix metalloproteinase-9 activity [J]. Neurol Res, 2010, 32(7): 756-762.
[14] Aboutaleb N, Shamsaei N, Rajabi H, et al. Protection of hippocampal CA1 neurons against ischemia/reperfusion injury by exercise preconditioning via modulation of Bax/Bcl-2 ratio and prevention of caspase-3 activation [J]. Basic Clin Neurosci, 2016, 7(1): 21-30.
[15] 朱路文,叶涛,吴孝军,等. 运动预处理对脑缺血再灌注大鼠血清炎症因子水平的影响[J]. 中国康复理论与实践, 2015, 21(1): 22-25.
[16] Zhu L, Ye T, Tang Q, et al. Exercise preconditioning regulates the toll-like receptor 4/nuclear factor-κB signaling pathway and reduces cerebral ischemia/reperfusion inflammatory injury: a study in rats [J]. J Stroke Cerebrovasc Dis, 2016, 25(11): 2770-2779.
[17] 吴孝军,朱路文,李宏玉,等. 运动预处理诱导脑缺血耐受机制的研究进展[J]. 中国康复理论与实践, 2015, 21(6): 657-661.
[18] 朱路文,叶涛,姜云飞,等. 电针预处理对大鼠脑缺血再灌注损伤后炎性因子及细胞凋亡的影响[J]. 中国康复理论与实践, 2016, 22(7): 765-768.
[19] He X, Mo Y, Geng W, et al. Role of Wnt/β-catenin in the tolerance to focal cerebral ischemia induced by electroacupuncture pretreatment [J]. Neurochem Int, 2016, 97: 124-132.
[20] Liu W, Wang X, Zheng Y, et al. Electroacupuncture inhibits inflammatory injury by targeting the miR 9 mediated NF-κB signaling pathway following ischemic stroke [J]. Mol Med Rep, 2016, 13(2): 1618-1626.
[21] Wu ZQ, Cui S, Zhu L, et al. Study on the mechanism of mTOR-mediated autophagy during electroacupuncture pretreatment against cerebral ischemic injury [J]. Evid Based Complement Alternat Med, 2016, 2016: 9121597.
[22] Wu Z, Zou Z, Zou R, et al. Electroacupuncture pretreatment induces tolerance against cerebral ischemia/reperfusion injury through inhibition of the autophagy pathway [J]. Mol Med Rep, 2015, 11(6): 4438-4446.
[23] Liu W, Shang G, Yang S, et al. Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway [J]. Int J Mol Med, 2016, 37(2): 309-318.
[24] Lakhan SE, Kirchgessner A, Tepper D, et al. Matrix metalloproteinases and blood-brain barrier disruption in acute ischemic stroke [J]. Front Neurol, 2013, 4: 32.
[25] Menezes MJ, McClenahan FK, Leiton CV, et al. The extracellular matrix protein laminin α2 regulates the maturation and function of the blood-brain barrier [J]. J Neurosci, 2014, 34(46): 15260-15280.
[26] 胡晓松,周东,唐瑜,等. 层粘连蛋白及基质金属蛋白酶-9在大鼠局灶性脑缺血梗死区周围的表达及意义[J]. 华西医学, 2013, 28(5): 651-654.

运动或电针预处理对脑梗死大鼠神经功能缺损及层粘连蛋白表达的影响

Effects of Exercise or Electroacupuncture Preconditioning on Neurological Deficits and Expression of Laminin in Rats with Cerebral Infarction

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	周治宁, 周容, 肖燕文, 王博文, 吕娇娇, 刘宇. 多靶区经颅直流电刺激对健康成年人工作记忆-姿势控制双任务表现的影响[J]. 《中国康复理论与实践》, 2024, 30(1): 21-28.
[2]	陈珺雯, 陈谦, 陈程, 李淑月, 刘玲玲, 吴存书, 龚翔, 鲁俊, 许光旭. 改良八段锦身体活动对脑卒中患者心肺功能、运动功能和日常生活活动能力的效果[J]. 《中国康复理论与实践》, 2024, 30(1): 74-80.
[3]	魏梦力, 钟亚平, 周易文, 桂辉贤, 关烨明, 于婷婷. 单侧前交叉韧带重建患者步行双侧下肢肌肉协同模式差异[J]. 《中国康复理论与实践》, 2024, 30(1): 95-104.
[4]	张冠聪, 黄秋晨, 顾蕊, 刘四海, 胡春英, 刘克敏. 不同神经肌肉训练方法对早期膝骨关节炎患者疼痛和运动功能效果的比较[J]. 《中国康复理论与实践》, 2023, 29(9): 1090-1097.
[5]	孙藤方, 任梦婷, 杨琳, 王耀霆, 王红雨, 闫兴洲. 高压氧治疗联合重复外周磁刺激干预脑卒中患者踝运动功能和平衡能力的效果[J]. 《中国康复理论与实践》, 2023, 29(8): 875-881.
[6]	王亚楠, 刘西花. 脑卒中偏瘫患者主观和客观平衡功能测量的相关性及预测效能[J]. 《中国康复理论与实践》, 2023, 29(8): 890-895.
[7]	魏晓微, 杨剑, 魏春艳, 贺启令. 学校环境下适应性体育课程促进智力与发展性残疾儿童心理运动发展的系统综述[J]. 《中国康复理论与实践》, 2023, 29(8): 910-918.
[8]	王海云, 王寅, 周信杰, 何爱群. 基于“中枢-外周-中枢”理论的经颅直流电刺激结合针刺干预脑卒中患者中枢及上肢功能的效果[J]. 《中国康复理论与实践》, 2023, 29(8): 919-925.
[9]	陈怡婷, 王倩, 崔慎红, 李映彩, 张思鈺, 魏衍旭, 任慧, 冷军, 陈斌. 双侧序贯重复经颅磁刺激干预脑卒中患者上肢运动功能的效果[J]. 《中国康复理论与实践》, 2023, 29(8): 926-932.
[10]	武广艳, 张冉, 王璐, 于歌, 陈亚平. 核心肌群运动控制训练对产后腹直肌分离的效果[J]. 《中国康复理论与实践》, 2023, 29(8): 948-953.
[11]	陈渔, 郭峰, 郭建瑞, 董彤彤, 夏雪莲. 镜像视觉反馈训练中大脑运动皮质激活的脑电特征研究[J]. 《中国康复理论与实践》, 2023, 29(8): 967-976.
[12]	李子怡, 宋为群, 杜巨豹, 曹光磊, 张艳明, 李冉. 运动表象训练对膝关节单髁置换术后膝关节功能的效果[J]. 《中国康复理论与实践》, 2023, 29(7): 745-749.
[13]	唐流泉, 彭兴云. 血流限制下运动对绝经后慢性阻塞性肺疾病患者脆性骨折部分危险因素的疗效[J]. 《中国康复理论与实践》, 2023, 29(7): 761-769.
[14]	韩亚兵, 刘少青, 李新通, 梁馨文, 罗敬, 李婷, 潘玮敏. 髋部神经肌肉训练对女子足球运动员前交叉韧带损伤风险的影响[J]. 《中国康复理论与实践》, 2023, 29(7): 770-776.
[15]	李芳, 霍速, 杜巨豹, 刘秀贞, 李小爽, 宋为群. 经颅直流电刺激联合任务导向性康复训练对脊髓损伤大鼠前肢运动障碍的效果[J]. 《中国康复理论与实践》, 2023, 29(7): 777-781.