信号转导接头蛋白2和输入蛋白5表达对骨骼肌蛋白质周转的影响

doi:10.3969/j.issn.1006-9771.2018.05.009

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (5): 535-538.doi: 10.3969/j.issn.1006-9771.2018.05.009

信号转导接头蛋白2和输入蛋白5表达对骨骼肌蛋白质周转的影响

刘雪云¹, 徐守宇²

1.浙江中医药大学附属第三医院,浙江杭州市 310000;
2.浙江中医药大学,浙江杭州市 310053

收稿日期:2017-12-12 修回日期:2018-02-12 出版日期:2018-05-25 发布日期:2018-05-24
通讯作者: 徐守宇。E-mail: 2857314214@qq.com或joshuu@med.juntendo.ac.jp
作者简介:刘雪云(1989-),女,汉族,浙江杭州市人,硕士研究生,主要研究方向：骨骼肌肉康复。通讯作者：徐守宇,主任医师,教授,硕士研究生导师,主要研究方向：骨骼肌肉与神经康复。
基金资助:
1.浙江省自然科学基金项目(No. LY12H17003); 2.国家中医药管理局中医康复服务能力规范化建设项目(No. [2016]130)

Effect of Signal-transducing Adaptor Protein 2 and Importin 5 Expression on Skeletal Muscle Protein Turnover

LIU Xue-yun¹, XU Shou-yu²

1. The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310000, China;
2. Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China

Received:2017-12-12 Revised:2018-02-12 Published:2018-05-25 Online:2018-05-24
Contact: XU Shou-yu. E-mail: 2857314214@qq.com or joshuu@med.juntendo.ac.jp
Supported by:
Supported by Zhejiang Natural Science Foundation (No. LY12H17003) and State Administration of Traditional Chinese Medicine Standardization Project of Traditional Chinese Medical Rehabilitation Service (No. [2016]130)

摘要/Abstract

摘要： 目的探讨信号转导接头蛋白2 (STAP2)和输入蛋白5 (Ipo5)表达对骨骼肌蛋白质周转的影响。方法 12只雄性Sprague-Dawley大鼠随机分入对照组(n=6)和模型组(n=6)。对照组不处理,模型组采用阻血诱导大鼠骨骼肌肥大。体外培养两组比目鱼肌细胞,模型组细胞用shRNA慢病毒感染细胞,先后实现基因STAP2、Ipo5沉默、过表达和功能挽救。Western blotting检测两组细胞STAP2、Ipo5和myostatin蛋白表达。结果模型组较对照组STAP2表达显著上升(t=-11.786, P<0.001),Ipo5和myostatin表达下降(t>14.039, P<0.001);在对照组、模型组、沉默组、过表达组、挽救组中,myostatin表达随STAP2表达降低而升高,随Ipo5表达升高而升高。结论 STAP2表达上调和Ipo5表达下降可促进蛋白质合成。

关键词: 骨骼肌, 蛋白质周转, 信号转导接头蛋白2, 输入蛋白5, 大鼠

Abstract: Objective To explore the expression of signal-transducing adaptor protein 2 (STAP2) and importin 5 (Ipo5), and the effect of them on skeletal muscle protein turnover. Methods Twelve male Sprague-Dawley rats were divided into control group (n=6) and model group (n=6). The model group established skeletal muscle hypertrophy induced by blood flow restriction. The soleus muscle cells in both groups were cultured in vitro, and those in the model group infected with shRNA lentivirus to make the expression of STAP2 and Ipo5 silencing, over-expression and gene function salvage finally. The expression of STAP2, Ipo5 and myostatin were determined with Western blotting in soleus muscle in both groups.Results The expression of STAP2 increased (t=-11.786, P<0.001), while the expression of Ipo5 and myostatin decreased (t>14.039, P<0.001) in the model groups compared with those in the control group. Under the control group and the model group, and target genes silencing group, overexpressing group and rescuing group, the expression of myostatin increased with STAP2 decrease and Ipo5 increase.Conclusion The expression of STAP2 increasing and Ipo5 decreasing would promote the protein synthesis.

Key words: skeletal muscle, protein turnover, signal-transducing adaptor protein 2, importin 5, rats

中图分类号:

R685

刘雪云, 徐守宇. 信号转导接头蛋白2和输入蛋白5表达对骨骼肌蛋白质周转的影响[J]. 《中国康复理论与实践》, 2018, 24(5): 535-538.

LIU Xue-yun, XU Shou-yu. Effect of Signal-transducing Adaptor Protein 2 and Importin 5 Expression on Skeletal Muscle Protein Turnover[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(5): 535-538.

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信号转导接头蛋白2和输入蛋白5表达对骨骼肌蛋白质周转的影响

Effect of Signal-transducing Adaptor Protein 2 and Importin 5 Expression on Skeletal Muscle Protein Turnover

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