Ski对脂多糖诱导大鼠星形胶质细胞炎症因子释放的影响

doi:10.3969/j.issn.1006-9771.2018.07.008

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (7): 802-806.doi: 10.3969/j.issn.1006-9771.2018.07.008

Ski对脂多糖诱导大鼠星形胶质细胞炎症因子释放的影响

王明^{1, 2}, 杨新乐¹, 陈铁戈^{1, 2}, 张东亮^{1, 2}, 巩朝阳^{1, 2}, 向高^{1, 2}, 刘开鑫^{1, 2}, 张海鸿¹

1.兰州大学第二医院骨科,甘肃兰州市 730030;
2.兰州大学甘肃省骨关节疾病研究重点实验室,甘肃兰州市 730030

收稿日期:2018-04-19 修回日期:2018-05-17 出版日期:2018-07-25 发布日期:2018-08-01
通讯作者: 张海鸿。E-mail: zhanghaihong1968@sina.com
作者简介:王明(1993-),男,汉族,陕西咸阳市人,硕士研究生,主要研究方向：脊柱脊髓损伤。通讯作者：张海鸿,男,主任医师,副教授,硕士研究生导师,主要研究方向：脊柱脊髓损伤。
基金资助:
1.甘肃省自然科学基金项目(No. 17JR5RA238); 2.兰州大学第二医院“萃英科技创新”基金项目(No. CY2017-MS06)

Role of Ski in Inflammatory Factor Release in Activated Astrocytes

WANG Ming^{1, 2}, YANG Xin-le¹, CHEN Tie-ge^{1, 2}, ZHANG Dong-liang^{1, 2}, GONG Zhao-yang^{1, 2}, XIANG Gao^{1, 2}, LIU Kai-xin^{1, 2}, ZHANG Hai-hong¹

1. Department of Orthopedics, the Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China;
2. Lanzhou University Key Laboratory of Orthopedics of Gansu Province, Lanzhou, Gansu 730030, China

Received:2018-04-19 Revised:2018-05-17 Published:2018-07-25 Online:2018-08-01
Contact: ZHANG Hai-hong. E-mail: zhanghaihong1968@sina.com
Supported by:
Supported by Gansu Natural Science Foundation (No. 17JR5RA238) and the Second Hospital of Lanzhou University Funding (No. CY2017-MS06)

摘要/Abstract

摘要： 目的探讨Ski对激活星形胶质细胞炎症因子分泌的影响。方法从3日龄Sprague-Dawley大鼠大脑皮质分离星形胶质细胞,分为空白对照组、阴性对照组、siRNA组,siRNA组沉默Ski基因。48 h后,采用Western blotting和免疫荧光染色检测Ski表达;再以脂多糖激活星形胶质细胞24 h。ELISA检测各组细胞中肿瘤坏死因子-α (TNF-α)和白细胞介素-1β (IL-1β)的浓度。结果 siRNA组Ski蛋白表达显著降低(P<0.001);激活后,TNF-α、IL-1β浓度显著减少(P<0.001)。结论 Ski可能参与星形胶质细胞炎症反应。

关键词: Ski, RNA干扰, 脂多糖, 炎症, 星形胶质细胞, 大鼠

Abstract: Objective To investigate the effect of Ski on the secretion of inflammatory cytokines from activated astrocytes. Methods Astrocytes were obtained from cerebral cortex of a three-day old Sprague-Dawley rat and cultured in vitro. They were divided into blank group, control group and siRNA group. The Ski gene was silenced in siRNA group. The expression of Ski was tested with Western blotting and immunofluorescence 48 hours later. Then the astrocytes were stimulated with lipopolysaccharide for 24 hours. The secretion of tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in activated astrocytes was detected with ELISA. Results The expression of Ski protein reduced in the siRNA group (P<0.001), as well as the secretion of TNF-α and IL-1β (P<0.001). Conclusion Ski may play a role in inflammatory response of astrocyte.

Key words: Ski, RNA interference, lipopolysaccharide, inflammation, astrocytes, rats

中图分类号:

R741

王明, 杨新乐, 陈铁戈, 张东亮, 巩朝阳, 向高, 刘开鑫, 张海鸿. Ski对脂多糖诱导大鼠星形胶质细胞炎症因子释放的影响[J]. 《中国康复理论与实践》, 2018, 24(7): 802-806.

WANG Ming, YANG Xin-le, CHEN Tie-ge, ZHANG Dong-liang, GONG Zhao-yang, XIANG Gao, LIU Kai-xin, ZHANG Hai-hong. Role of Ski in Inflammatory Factor Release in Activated Astrocytes[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(7): 802-806.

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Ski对脂多糖诱导大鼠星形胶质细胞炎症因子释放的影响

Role of Ski in Inflammatory Factor Release in Activated Astrocytes

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