黄角颗粒对脑缺血再灌注损伤大鼠炎性因子及凋亡相关蛋白的影响

doi:10.3969/j.issn.1006-9771.2018.08.005

《中国康复理论与实践》 ›› 2018, Vol. 24 ›› Issue (8): 893-899.doi: 10.3969/j.issn.1006-9771.2018.08.005

黄角颗粒对脑缺血再灌注损伤大鼠炎性因子及凋亡相关蛋白的影响

潘宋斌¹, 万琳², 邵卫¹, 唐坤¹, 姚汉云¹

1.武汉市第一医院神经内科,湖北武汉市 430022;
2.武汉市中医医院内分泌科,湖北武汉市 430014

收稿日期:2018-04-12 修回日期:2018-07-02 出版日期:2018-08-25 发布日期:2018-08-20
通讯作者: 万琳。E-mail: niuniu9804@163.com
作者简介:潘宋斌(1980-),男,汉族,湖北武汉市人,硕士,主治医师,主要研究方向：运动障碍疾病和脑血管疾病的中西医结合治疗。通讯作者：万琳(1980-),女,汉族,湖北武汉市人,硕士,主治医师,主要研究方向：中西医结合治疗糖尿病及其并发症。
基金资助:
武汉市卫计委科研项目(No. WZ16D04)

Effect of Huangjiao Granule on Inflammatory Factors and Apoptosis-related Proteins in Rats with Cerebral Ischemia-reperfusion Injury

PAN Song-bin¹, WAN Lin², SHAO Wei¹, TANG Kun¹, YAO Han-yun¹

1. Department of Neurology, Wuhan No.1 Hospital, Wuhan, Hubei 430022, China;
2. Department of Endocrinology, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430014, China

Received:2018-04-12 Revised:2018-07-02 Published:2018-08-25 Online:2018-08-20
Contact: WAN Lin. E-mail: niuniu9804@163.com

摘要/Abstract

摘要： 目的探讨黄角颗粒对脑缺血再灌注损伤大鼠炎性因子及凋亡相关蛋白的影响。方法 SPF级Sprague-Dawley健康雄性大鼠40只随机分为假手术组、模型组和黄角颗粒组,每组10只,余10只备用。采用线栓法构建大鼠脑缺血2 h再灌注模型,其中假手术组和模型组在假手术前或缺血再灌注前30 min予生理盐水10 ml/kg灌胃,黄角颗粒组在缺血再灌注前30 min予黄角颗粒溶液10 ml/kg (生药含量1 g/ml)灌胃。再灌注24 h后,Longa评分法对各组大鼠进行神经功能评分,TTC染色检测各组脑梗死体积百分比,HE染色观察各组脑组织病理形态,TUNEL检测各组脑细胞凋亡,ELISA检测各组血清中白细胞介素(IL)-1β、IL-6和肿瘤坏死因子α (TNF-α)的含量,Western blotting检测各组大鼠脑组织中cleaved-caspase 3、cleaved-caspase 9、Bax和Bcl-2蛋白的表达。结果与模型组相比,黄角颗粒组神经功能评分显著降低(P<0.001),脑梗死体积百分比和脑细胞凋亡率降低(P<0.05);血清中IL-1β、IL-6和TNF-α的表达水平降低(P<0.05);脑组织中cleaved-caspase 3、cleaved-caspase 9和Bax的表达均降低(P<0.05),Bcl-2的表达升高(P<0.05)。结论黄角颗粒对脑缺血再灌注损伤大鼠的保护作用与抑制炎症反应和减少细胞凋亡相关。

关键词: 脑缺血再灌注损伤, 黄角颗粒, 炎性因子, 凋亡相关蛋白, 大鼠

Abstract: Objective To investigate the effect of Huangjiao granule on inflammatory factors and apoptosis-related proteins in rats with cerebral ischemia-reperfusion injury.Methods A total of 40 Sprague-Dawley rats were divided into sham operation group, model group and Huangjiao granule group, with ten rats in each group, and ten rats standby. The cerebral ischemia for two hours and reperfusion model was established by suture method. The sham operation group and the model group were given saline 10 ml/kg intragastrically 30 minutes before operation. The Huangjiao granule group was given Huangjiao granule solution 10 ml/kg (the content of crude drug was 1 g/ml) intragastrically 30 minutes before ischemia-reperfusion. Longa scoring method was used to evaluate the neurological function score 24 hours after reperfusion, while the percentage of cerebral infarction volume was detected by TTC staining, the pathological morphology of brain tissue was observed by HE staining, the cell apoptosis of brain tissue was detected with TUNEL, the levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor α (TNF-α) in serum were detected by ELISA, the expression of cleaved-caspase 3, cleaved-caspase 9, Bax and Bcl-2 proteins of brain tissue was evaluated by Western blotting.Results Compared with the model group, the neurological score decreased, the percentage of cerebral infarction volume and the apoptosis rate of the brain tissue decreased in Huangjiao granule group (P<0.05). The levels of IL-1β, IL-6 and TNF-α in serum and the expression of cleaved-caspase 3, cleaved-caspase 9 and Bax proteins of brain tissues significantly decreased (P<0.05), and the expression of Bcl-2 protein of brain tissues inreased in Huangjiao granule group (P<0.05).Conclusion The protective effect of Huangjiao granule on rats with cerebral ischemia-reperfusion injury may be related to the inhibition of inflammatory response and the reduction of apoptosis.

Key words: cerebral ischemia-reperfusion injury, Huangjiao granule, inflammatory factors, apoptosis-related proteins, rats

中图分类号:

R743.3

潘宋斌, 万琳, 邵卫, 唐坤, 姚汉云. 黄角颗粒对脑缺血再灌注损伤大鼠炎性因子及凋亡相关蛋白的影响[J]. 《中国康复理论与实践》, 2018, 24(8): 893-899.

PAN Song-bin, WAN Lin, SHAO Wei, TANG Kun, YAO Han-yun. Effect of Huangjiao Granule on Inflammatory Factors and Apoptosis-related Proteins in Rats with Cerebral Ischemia-reperfusion Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(8): 893-899.

参考文献

[1] 彭智远,刘旺华,曹雯. 脑缺血再灌注损伤细胞凋亡机制的研究进展[J]. 中华中医药学刊, 2017, 35(8): 1957-1961.
[2] Love S, Miners JS.Cerebrovascular disease in ageing and Alzheimer's disease[J]. Acta Neuropathol, 2016, 131(5): 645-658.
[3] Chen FY, Fang XY, Zhang H.Effect of polydatin on expression of p53 and Notch1 in brain tissue of ischemic cerebrovascular disease[J]. J Biol Regul Homeost Agents, 2018, 32(1): 133-138.
[4] Gao XJ, Xie GN, Liu L, et al.Sesamol attenuates oxidative stress, apoptosis and inflammation in focal cerebral ischemia/reperfusion injury[J]. Exp Ther Med, 2017, 14(1): 841-847.
[5] Ahmad M, Graham SH.Inflammation after stroke: mechanisms and therapeutic approaches[J]. Transl Stroke Res, 2010, 1(2): 74-84.
[6] Broughton BR, Reutens DC, Sobey CG.Apoptotic mechanisms after cerebral ischemia[J]. Stroke, 2009, 40(5): e331-e339.
[7] Yang Y, Zhang ZX, Lian D, et al.IL-37 inhibits IL-18-induced tubular epithelial cell expression of pro-inflammatory cytokines and renal ischemia-reperfusion injury[J]. Kidney Int, 2015, 87(2): 396-408.
[8] Chen S, Yin ZJ, Jiang C, et al.Asiaticoside attenuates memory impairment induced by transient cerebral ischemia-reperfusion in mice through anti-inflammatory mechanism[J]. Pharmacol Biochem Behav, 2014, 122: 7-15.
[9] 陈国华,常学辉,吴辉. 黄角颗粒对MCAO大鼠血浆SOD、MDA、TNF-α、NO含量的影响[J]. 中国中医急症, 2006, 15(1): 75-77.
[10] 陈国华,吴辉,罗利俊. 黄角颗粒对MCAO大鼠神经细胞bc1-2、bax表达影响的实验研究[J]. 中国中医急症, 2006, 15(2): 178-179.
[11] 陈媛,黄晓松,吴海金,等. 葛根素对大鼠脑缺血再灌注损伤后JAK2/STAT3信号通路的影响[J]. 湖南中医杂志, 2017, 33(4): 144-147.
[12] Longa EZ, Weinstein PR, Carlson S, et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1): 84-91.
[13] 刘秀平,许栋明,王文,等. 炎症反应影响脑缺血再灌注损伤的研究进展[J]. 中国康复理论与实践, 2009, 15(11): 1041-1043.
[14] 陈勇军,汤永红,龙慧. 促红细胞生成素对大鼠脑缺血再灌注损伤后炎症反应的影响[J]. 中国康复理论与实践, 2008, 14(8): 729-730.
[15] 王萍,张密霞,庄朋伟,等. 脑缺血再灌注损伤的炎症反应机制研究进展[J]. 天津中医药大学学报, 2014, 33(5): 317-320.
[16] 段乐,白利群,田苗苗,等. 炎症反应在脑缺血中作用机制研究进展[J]. 中华实用诊断与治疗杂志, 2013, 27(9): 837-839.
[17] Caso JR, Moro MA, Lorenzo P, et al.Involvement of IL-1beta in acute stress-induced worsening of cerebral ischaemia in rats[J]. Eur Neuropsychopharmacol, 2007, 17(9): 600-607.
[18] Di Paola R, Genovese T, Impellizzeri D, et al.The renal injury and inflammation caused by ischemia-reperfusion are reduced by genetic inhibition of TNF-αR1: a comparison with infliximab treatment[J]. Eur J Pharmacol, 2013, 700(1-3): 134-146.
[19] Basic KV, Simundic AM, Nikolac N, et al.Pro-inflammatory and anti-inflammatory cytokines in acute ischemic stroke and their relation to early neurological deficit and stroke outcome[J]. Clin Biochem, 2008, 41(16): 1330-1334.
[20] 葛建彬,卢红建,宋新建,等. 枸杞多糖对小鼠脑缺血再灌注损伤的保护作用及其抑制NF-κB,TNF-α,IL-6和IL-1β表达的机制[J]. 中国中药杂志, 2017, 42(2): 326-331.
[21] Chen CM, Wu CT, Yang TH, et al.Preventive effect of low intensity pulsed ultrasound against experimental cerebral ischemia/reperfusion injury via apoptosis reduction and brain-derived neurotrophic factor induction[J]. Sci Rep, 2018, 8(1): 5568.
[22] 朱路文,叶涛,姜云飞,等. 电针预处理对大鼠脑缺血再灌注损伤后炎性因子及细胞凋亡的影响[J]. 中国康复理论与实践, 2016, 22(7): 765-768.
[23] 陈红兵,王鲁民,郭云良. Caspase与脑缺血研究进展[J]. 中国全科医学, 2003, 6(10): 874-875.
[24] 李小艳,包素珍. Caspase家族的研究进展[J]. 内蒙古中医药, 2012, 31(2): 107-107.
[25] Fan TJ, Han LH, Cong RS, et al.Caspase family proteases and apoptosis[J]. Acta Biochim Biophys Sin, 2005, 37(11): 719-727.
[26] 兰晶,潘敬芳. 姜黄素对脑缺血再灌注损伤大鼠PI3K/AKT/mTOR的影响[J]. 重庆医学, 2017, 46(1): 36-39.

黄角颗粒对脑缺血再灌注损伤大鼠炎性因子及凋亡相关蛋白的影响

Effect of Huangjiao Granule on Inflammatory Factors and Apoptosis-related Proteins in Rats with Cerebral Ischemia-reperfusion Injury

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