有氧运动对脑小血管病大鼠执行功能的效果

doi:10.3969/j.issn.1006-9771.2019.03.001

《中国康复理论与实践》 ›› 2019, Vol. 25 ›› Issue (3): 249-254.doi: 10.3969/j.issn.1006-9771.2019.03.001

• 专题 • 下一篇

有氧运动对脑小血管病大鼠执行功能的效果

王茹^1,2, 刘楠¹, 李卫萍², 赵弘轶¹, 杜菊梅², 黄勇华¹

1.中国人民解放军总医院第七医学中心神经内科，北京市 100700
2.陕西中医药大学第二附属医院，陕西咸阳市 712000

收稿日期:2018-11-26 修回日期:2019-01-16 出版日期:2019-03-25 发布日期:2019-04-02
通讯作者: 黄勇华，E-mail： huangyh@163.com
作者简介:王茹(1991-)，女，汉族，陕西延安市人，硕士，主要研究方向：脑血管病。通讯作者：黄勇华(1969-)，男，广东梅州市人，博士，主要研究方向：脑血管疾病及睡眠障碍。
基金资助:
国家自然科学基金青年项目(No. 81601035)

Effect of Aerobic Exercise on Behavioral Function in Rats with Cerebral Small Vessel Disease

WANG Ru^1,2, LIU Nan¹, LI Wei-ping², ZHAO Hong-yi¹, DU Ju-mei², HUANG Yong-hua¹

1.Department of Neurology, the Seventh Medical Center of PLA General Hospital, Beijing 100700, China
2.Department of Neurology, Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712000, China

Received:2018-11-26 Revised:2019-01-16 Published:2019-03-25 Online:2019-04-02
Contact: HUANG Yong-hua, E-mail： huangyh@163.com
Supported by:
National Natural Science Foundation of China (Youth) (No. 81601035)

摘要/Abstract

摘要： 目的探讨有氧运动改善脑小血管病大鼠认知执行能力的效果及作用机制。方法 8周龄雄性Sprague-Dawley大鼠随机分为假手术组(n = 16)、模型组(n = 16)和游泳组(n = 16)。双侧颈动脉结扎法造模。4周游泳锻炼后，各组行挖穴测试。Western blotting检测海马脑源性神经营养因子(BDNF)和原肌球蛋白受体激酶B (TrkB)蛋白水平。高尔基染色法观察海马神经元树突及突触棘的发育情况。免疫荧光法检测海马齿状回Ki67、双皮质素(DCX)和Neun的表达。结果与模型组相比，游泳组挖穴能力更强(P < 0.05)，海马组织中BDNF和TrkB蛋白水平更高(P < 0.05)，海马齿状回Ki67/DCX和Neun阳性细胞表达率更高(P < 0.05)，海马区神经元树突范围更大，突触棘更长，神经元发育更好(P < 0.05)。结论有氧运动通过BDNF/TrkB信号通路促进海马神经元的增殖分化，增加Ki67/DCX和Neun表达，促进海马神经元发育，改善脑小血管病大鼠执行功能。

关键词: 脑小血管病, 有氧运动, 海马, 执行功能, 脑源性神经营养因子, 原肌球蛋白受体激酶B, 大鼠

Abstract: Objective To explore the effect of aerobic exercise on behavioral function in rats with cerebral small vessel disease and its mechanism. Methods Eight-week-old male Sprague-Dawley rats were randomly divided into sham group (n = 16), model group (n = 16) and swimming group (n = 16). The model was developed with bilateral common carotid artery ligation. They were assessed with burrowing test after four weeks of swimming exercise. The levels of brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) protein in hippocampus were detected with Western blotting. The development of dendrites and synaptic spines in hippocampal neurons was observed with Golgi staining. The expression of Ki67, doublecortin (DCX) and Neun in hippocampal dentate gyrus was detected with immunofluorescence. Results Compared with the model group, the burrowing ability improved in the swimming group (P < 0.05), with increase of levels of BDNF and TrkB in hippocampus (P < 0.05), Ki67/DCX and Neun positive cells in hippocampal dentate gyrus (P < 0.05), and extension of dendrites and length of synaptic spine in hippocampal neurons (P < 0.05). Conclusion Aerobic exercise may promote the proliferation and differentiation of hippocampal neurons through BDNF/TrkB signaling pathway, expression of Ki67/DCX and Neun and development of hippocampal neurons, to improve behavioral function in rats with cerebral small vessel disease.

Key words: cerebral small vessel disease, aerobic exercise, hippocampus, behavioral function, brain-derived neurotrophic factor, tropomyosin receptor kinase B, rats

中图分类号:

R743

王茹, 刘楠, 李卫萍, 赵弘轶, 杜菊梅, 黄勇华. 有氧运动对脑小血管病大鼠执行功能的效果[J]. 《中国康复理论与实践》, 2019, 25(3): 249-254.

WANG Ru, LIU Nan, LI Wei-ping, ZHAO Hong-yi, DU Ju-mei, HUANG Yong-hua. Effect of Aerobic Exercise on Behavioral Function in Rats with Cerebral Small Vessel Disease[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2019, 25(3): 249-254.

参考文献

1 SudlowC L, WarlowC P. Comparable studies of the incidence of stroke and its pathological types: results from an international collaboration. International Stroke Incidence Collaboration [J]. Stroke, 1997, 28(3): 491-499.
2 FujinoY, AmimotoK, SugimotoS, et al. Relationship of white matter lesions and severity of pushing behavior after stroke [J]. J Phys The Sci, 2017, 29(12): 2116-2120.
3 GorelickP B, ScuteriA, BlackS E, et al. Vascular contributions to cognitive impairment and dementia: a statement for healthcare professionals from the American Heart Association/American Stroke Association [J]. Stroke, 2011, 42(9): 2672-2713.
4 周婷婷,李晓红,梁战华. 脑小血管病所致血管性认知功能障碍研究进展[J]. 中国卒中杂志, 2018, 13(9): 896-899.
5 StimpsonN J, DavisonG, JavadiA H. Joggin' the noggin: towards a physiological understanding of exercise-induced cognitive benefits [J]. Neurosci Biobehav Rev, 2018, 5(88): 177-186.
6 OlivierD, GauthierC J, FraserS A, et al. Higher levels of cardiovascular fitness are associated with better executive function and prefrontal oxygenation in younger and older women [J]. Front Hum Neurosci, 2015, 9: 66.
7 MarosiK, MattsonM P. BDNF mediates adaptive brain and body responses to energetic challenges [J]. Trends Endocrinol Metab, 2014, 25(2): 89-98.
8 HuW, FengZ, XuJ, et al. Brain-derived neurotrophic factor modified human umbilical cord mesenchymal stem cells-derived cholinergic-like neurons improves spatial learning and memory ability in Alzheimer's disease rats [J]. Brain Res, 2018, 8993(18): 30660-30667.
9 LiuP Z , RobinN . Exercise-mediated neurogenesis in the hippocampus via BDNF [J]. Front Neurosci, 2018, 12(7): 52-58.
10 ManjuS, VignoliB, CanossaM, et al. Neurobiology of local and intercellular BDNF signaling [J]. Pflugers Arch, 2017, 469(5): 593-610.
11 GuoW, NagappanG, LuB. Differential effects of transient and sustained activation of BDNF-TrkB signaling [J]. Dev Neurobiol, 2018, 78(7): 647-657.
12 NokiaM S, LensuS, AhtiainenJ P, et al. Physical exercise increases adult hippocampal neurogenesis in male rats provided it is aerobic and sustained [J]. J Physiol, 2016, 594(7): 1855-1873.
13 AnthonyS S, DateI, YasuharaT. Limiting exercise inhibits neuronal recovery from neurological disorders [J]. Brain Circ, 2017, 3(3): 124-129.
14 PattenA R, YauS Y, FontaineC J, et al. The benefits of exercise on structural and functional plasticity in the rodent hippocampus of different disease models [J]. Brain Plast, 2015, 1(1): 97-127.
15 LuB, NagappanG, LuY. BDNF and synaptic plasticity, cognitive function, and dysfunction [J]. Handb Exp Pharmacol, 2014, 220(9): 223-250.
16 倪俊,徐运. 脑小血管病转化医学研究中国专家共识[J]. 中国卒中杂志, 2018, 13(8): 853-870.
17 Gegentonglaga, YoshizatoH, HiguchiY, et al. Variable alteration of regional tissue oxygen pressure in rat hippocampus by acute swimming exercise [J]. Life Sci, 2013, 93(21): 773-777.
18 CechellaJ L, LeiteM R, RosarioA R, et al. Diphenyl diselenide-supplemented diet and swimming exercise enhance novel object recognition memory in old rats [J]. Age, 2014, 36(4): 9666-9668.
19 DeaconR M. Burrowing in rodents: a sensitive method for detecting behavioral dysfunction [J]. Nat Protoc, 2006, 1(1): 118-121.
20 NkomozepiP, MazengenyaP, IhunwoA O. Age-related changes in Ki-67 and DCX expression in the BALB/ c mouse (Mus Musculus) brain [J]. Int J Dev Neurosci, 2019, 72(5): 36-47.
21 KaraN, NarayananS, BelmakerR H, et al. Chronic lithium treatment enhances the number of quiescent neural progenitors but not the number of DCX-positive immature neurons [J]. Int J Neuropsychopharmacol, 2015, 18(7): pyv003.
22 XieQ, ChengJ, PanG, et al. Treadmill exercise ameliorates focal cerebral ischemia/reperfusion-induced neurological deficit by promoting dendritic modification and synaptic plasticity via upregulating caveolin-1/VEGF signaling pathways [J]. Exp Neurol, 2018, 313(5): 60-78.
23 VossM W, VivarC, KramerA F, et al. Bridging animal and human models of exercise-induced brain plasticity [J]. Trends Cogn Sci, 2013, 17(10): 525-544.
24 张新安. 有氧运动改善血管性痴呆大鼠认知功能的皮质机制研究:随机对照实验方案[J]. 中国组织工程研究, 2016, 20(46): 6943-6949.
25 蓝永生,张殿禹,蒋艳杰. 不同强度有氧+力量混合运动对老年大鼠海马神经发生的影响[J]. 中国老年学杂志, 2018, 38(17): 4249-4251.
26 EtnierJ L, NowellP M, LandersD M, et al. A meta-regression to examine the relationship between aerobic fitness and cognitive performance [J]. Brain Res Rev, 2006, 52(1): 119-130.
27 MakanyaA N, HlushchukR, DjonovV G. Intussusceptive angiogenesis and its role in vascular morphogenesis, patterning, and remodeling [J]. Angiogenesis, 2009, 12(2): 113-123.
28 BaileyD M, EvansK A, McenenyJ, et al. Exercise‐induced oxidative-nitrosative stress is associated with impaired dynamic cerebral autoregulation and blood-brain barrier leakage [J]. Exp Physiol, 2011, 96(11): 1196-1207.
29 GrabanJ, HlavacovaN, JezovaD. Increased gene expression of selected vesicular and glial glutamate transporters in the frontal cortex in rats exposed to voluntary wheel running [J]. J Physiol Pharmacol, 2017, 68(5): 709-714.
30 VecchioL M, MengY, XhimaK, et al. The neuroprotective effects of exercise: maintaining a healthy brain throughout aging [J]. Brain Plast, 2018, 4(1): 17-52.
31 H?kanssonK, LedreuxA, DaffnerK, et al. BDNF responses in healthy older persons to 35 minutes of physical exercise, cognitive training, and mindfulness: associations with working memory function [J]. J Alzheimers Dis, 2017, 55(2): 645-657.
32 孙竹梅,李建民,王国立,等. 有氧运动预处理对全脑缺血再灌注大鼠再生相关因子的影响[J]. 中国康复理论与实践, 2016, 22(7): 759-764.
33 LinT W, TsaiS F, KuoY M. Physical exercise enhances neuroplasticity and delays Alzheimer's disease [J]. Brain Plast, 2018, 4(1): 95-110.
34 ThackerJ S, MiddletonL E, McIlroyW E, et al. The influence of an acute bout of aerobic exercise on cortical contributions to motor preparation and execution [J]. Physiol Rep, 2014, 2(10): 12178-12185.
35 LiZ, PengX, XiangW, et al. The effect of resistance training on cognitive function in the older adults: a systematic review of randomized clinical trials [J]. Aging Clin Exp Res, 2018, 13(10): 1007-1021.
36 MarshallR S, LazarR M. Pumps, aqueducts, and drought management: vascular physiology in vascular cognitive impairment [J]. Stroke, 2011, 42(1): 221-226.

[1]	宋以玲, 任园春, 朱飞龙, 匡冬青, 曹庆久, 林杨, 王芳. 注意缺陷多动障碍儿童粗大动作技能与执行功能发展的特点及关系[J]. 《中国康复理论与实践》, 2024, 30(1): 1-9.
[2]	闻嘉宁, 金秋艳, 张琦, 李杰, 司琦. 认知参与型身体活动对发展儿童青少年执行功能的效果：基于ICF的系统综述[J]. 《中国康复理论与实践》, 2024, 30(1): 44-53.
[3]	王俊宇, 杨永, 袁逊, 谢婷, 庄洁. 高强度间歇训练对健康儿童青少年执行功能效果的系统综述[J]. 《中国康复理论与实践》, 2023, 29(9): 1012-1020.
[4]	李芳, 霍速, 杜巨豹, 刘秀贞, 李小爽, 宋为群. 经颅直流电刺激联合任务导向性康复训练对脊髓损伤大鼠前肢运动障碍的效果[J]. 《中国康复理论与实践》, 2023, 29(7): 777-781.
[5]	田俊夫, 杨剑. 物质滥用导致精神行为障碍有氧运动康复的健康和功能效益的系统综述[J]. 《中国康复理论与实践》, 2023, 29(4): 443-451.
[6]	李慕瑶, 王梅, 杜亮, 童露, 许三雄. 基于WHO-FICs架构有氧联合抗阻运动对老年冠心病并发高血压患者的效果[J]. 《中国康复理论与实践》, 2023, 29(3): 326-334.
[7]	罗兰, 李璐, 金沐. 氙气后处理对脊髓缺血再灌注损伤的效果：Akt信号通路和自噬机制[J]. 《中国康复理论与实践》, 2023, 29(2): 174-181.
[8]	黄志霖, 徐发邵, 施静, 黄淦, 刘梅芳, 张霞辉. 线栓法建立卒中后吞咽障碍的大鼠模型[J]. 《中国康复理论与实践》, 2023, 29(10): 1147-1153.
[9]	王宇, 贺刚, 黎东升, 马恺悦, 王超. 身体活动干预儿童青少年执行功能效果的系统综述[J]. 《中国康复理论与实践》, 2023, 29(1): 20-29.
[10]	秦彦强, 董浩, 孙迎春, 程先宽, 姚海江. 不同针刺方案对卒中后抑郁大鼠神经递质及相关炎性因子的影响[J]. 《中国康复理论与实践》, 2023, 29(1): 30-37.
[11]	陈婷婷,钟晓珂,温柔. 舞蹈活动改善儿童认知功能的系统综述[J]. 《中国康复理论与实践》, 2022, 28(8): 889-896.
[12]	缪培,张通,米海霞,张伟东. 不同线栓法复制局灶性脑缺血模型大鼠恢复期学习记忆能力的差异及其机制[J]. 《中国康复理论与实践》, 2022, 28(7): 789-796.
[13]	王琛,冷文武,王志鹏,陈瀚哲,张继,许明超,钟晓珂,蒋长好. 12周有氧运动对超重儿童抑制控制功能的影响[J]. 《中国康复理论与实践》, 2022, 28(6): 684-689.
[14]	陈小明,梁冠军,李明娣,王庆娟,苏敏. 有氧运动对注意缺陷多动障碍儿童核心症状和执行功能的影响[J]. 《中国康复理论与实践》, 2022, 28(6): 704-709.
[15]	周小珏,冯婧,庞日朝,刘捷,张安仁. 隔日限食减轻脊髓损伤大鼠炎症反应的芳香烃受体/细胞因子信号传导抑制因子2/核转录因子-κB信号通路机制[J]. 《中国康复理论与实践》, 2022, 28(5): 544-551.

有氧运动对脑小血管病大鼠执行功能的效果

Effect of Aerobic Exercise on Behavioral Function in Rats with Cerebral Small Vessel Disease

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0