电针对APP/PS1小鼠皮质区磷脂酰肌醇-3激酶/糖原合成酶激酶-3α通路相关蛋白表达和老年斑沉积的影响

doi:10.3969/j.issn.1006-9771.2020.09.009

《中国康复理论与实践》 ›› 2020, Vol. 26 ›› Issue (9): 1045-1051.doi: 10.3969/j.issn.1006-9771.2020.09.009

电针对APP/PS1小鼠皮质区磷脂酰肌醇-3激酶/糖原合成酶激酶-3α通路相关蛋白表达和老年斑沉积的影响

伍艳君¹,邬开会²,刘茜¹,王依滢¹,邱国平¹,徐进¹,盛华均¹,朱淑娟¹()

1.重庆医科大学神经科学研究中心,重庆医科大学人体解剖学教研室,重庆市400016
2.重庆市南川区人民医院,重庆市408400

收稿日期:2019-11-25 修回日期:2020-04-01 出版日期:2020-09-25 发布日期:2020-09-24
通讯作者: 朱淑娟 E-mail:shujuanzhu@cqmu.edu.cn
作者简介:伍艳君(1993-),女,汉族,重庆市人,硕士研究生,主要研究方向：针刺防治阿尔茨海默病应用基础研究。|朱淑娟(1975-),女,汉族,陕西西安市人,博士,副教授,硕士生导师,主要研究方向：神经生物学。
基金资助:
1.国家自然科学基金项目(81502161);2.重庆市科委项目(cstc2016jcyjA0229);3.重庆市渝中区科技计划项目(20150122)

Effect of Electroacupuncture on Expression of Phosphatidylinositol 3 Kinase/Glycogen Synthase Kinase 3 Alpha Pathway Related Proteins and Senile Plaque Deposition in Cortex of APP/PS1 Mice

WU Yan-jun¹,WU Kai-hui²,LIU Qian¹,WANG Yi-ying¹,QIU Guo-ping¹,XU Jin¹,SHENG Hua-jun¹,ZHU Shu-juan¹()

1. Department of Human Anatomy, Chongqing Medical University, Chongqing 400016, China
2. People's Hospital of Nanchuan, Chongqing 408400, China

Received:2019-11-25 Revised:2020-04-01 Published:2020-09-25 Online:2020-09-24
Contact: ZHU Shu-juan E-mail:shujuanzhu@cqmu.edu.cn
Supported by:
National Natural Science Foundation of China(81502161);National Science Foundation of CQCSTC(cstc2016jcyjA0229);Chongqing Yuzhong District Science and Technology Plan(20150122)

摘要/Abstract

摘要：

目的观察电针对APP/PS1双转基因小鼠皮质区磷脂酰肌醇-3激酶/糖原合成酶激酶-3α (PI3K/GSK3α)信号通路上相关蛋白的分布表达,以及老年斑沉积的影响。
方法基因鉴定后,3月龄雄性转基因小鼠随机分为模型组和电针组,野生型C57小鼠为对照组,每组6只。电针组电针百会(GV20)和双侧肾俞(BL23)14 d。治疗后,Western blotting和免疫组化法检测各组小鼠皮质区老年斑数量,PI3K亚单位P85α和P110α,以及GSK3α和pS^21-GSK3α等蛋白的分布和表达。
结果模型组皮质区老年斑数较对照组显著升高(P < 0.001);电针组老年斑数较模型组显著降低( P < 0.001)。各相关蛋白主要表达于皮质神经元胞质。与对照组相比,模型组pS ^21-GSK3α、P110α和P85α蛋白表达明显降低(P < 0.01),GSK3α蛋白表达升高( P < 0.05);与模型组相比,电针组pS ^21-GSK3α、P110α和P85α的蛋白表达明显升高(P < 0.01),GSK3α蛋白表达降低( P < 0.05)。
结论电针能调节APP/PS1双转基因小鼠皮质区PI3K/GSK3α通路相关蛋白表达,减少老年斑沉积。

关键词: 阿尔茨海默病, 电针, 磷脂酰肌醇-3激酶, 糖原合成酶激酶-3α, 信号通路, 小鼠

Abstract:

Objective To observe the effects of electroacupuncture (EA) on expression of phosphatidylinositol 3 kinase/glycogen synthase kinase 3 alpha (PI3K/GSK3α) signaling pathway related proteins in cortex of APP/PS1 double transgenic mice, and the senile plaque (SP) deposit.
Methods Three-month-old male APP/PS1 mice were randomly divided into model group (n = 6) and EA group (n = 6) after gene identification, and other six wild C57 mice were as control group. EA group accepted EA at Baihui (GV20) and bilateral Shenshu (BL23) for 14 days. Then, the amount of SP in cortex, the expression of P85α and P110α, subunits of PI3K, and GSK3α and pS^21-GSK3α, were detected with Western blotting and immunohistochemistry.
Results The SP increased in the model group compared with that of the control group (P < 0.001), and it decreased in EA group compared with that of the model group ( P < 0.001). All the proteins expressed in the cytoplasm of cortical neurons. The expression of pS ^21-GSK3α, P110α and P85α decreased in the model group compared with that of the control group (P < 0.01), while the expression of GSK3α increased ( P < 0.05). The expression of pS ^21-GSK3α, P110α and P85α increased in EA group compared with that of the model group (P < 0.01), while the expression of GSK3α decreased ( P < 0.05).
Conclusion EA may adjust the PI3K/GSK3α signaling pathway, to reduce the formation and deposition of SP in APP/PS1 double-transgenic mice.

Key words: Alzheimer's disease, electroacupuncture, phosphatidylinositol 3-kinase, glycogen synthase kinase-3α, signaling pathway, mice

伍艳君,邬开会,刘茜,王依滢,邱国平,徐进,盛华均,朱淑娟. 电针对APP/PS1小鼠皮质区磷脂酰肌醇-3激酶/糖原合成酶激酶-3α通路相关蛋白表达和老年斑沉积的影响[J]. 《中国康复理论与实践》, 2020, 26(9): 1045-1051.

WU Yan-jun,WU Kai-hui,LIU Qian,WANG Yi-ying,QIU Guo-ping,XU Jin,SHENG Hua-jun,ZHU Shu-juan. Effect of Electroacupuncture on Expression of Phosphatidylinositol 3 Kinase/Glycogen Synthase Kinase 3 Alpha Pathway Related Proteins and Senile Plaque Deposition in Cortex of APP/PS1 Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(9): 1045-1051.

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组别	n	老年斑	GSK3α	pS²¹-GSK3α	P110α	P85α
对照组	6	0	26.350±2.367	29.800±2.622	54.150±4.113	21.180±1.472
模型组	6	6.500±2.074^a	49.590±2.457^a	22.770±0.856^a	29.340±3.555^a	16.790±2.414^b
电针组	6	2.500±1.049^c	29.030±2.259^c	27.580±1.014^c	49.320±2.268^c	21.870±0.947^c
F值		24.310	186.7000	34.770	88.940	11.740
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	GSK3α	pS^21-GSK3α	P110α	P85α	pS^21-GSK3α/GSK3α
对照组	6	0.987±0.283	0.983±0.114	1.160±0.114	0.912±0.230	1.068±0.310
模型组	6	1.563±0.370^a	0.521±0.118^b	0.720±0.102^b	0.450±0.234^c	0.346±0.118^b
电针组	6	1.007±0.252^d	0.861±0.182^e	1.080±0.102^f	0.942±0.213^e	0.890±0.237^e
F值		6.062	18.800	28.200	8.565	18.050
P值		0.004	< 0.001	< 0.001	< 0.001	< 0.001

电针对APP/PS1小鼠皮质区磷脂酰肌醇-3激酶/糖原合成酶激酶-3α通路相关蛋白表达和老年斑沉积的影响

Effect of Electroacupuncture on Expression of Phosphatidylinositol 3 Kinase/Glycogen Synthase Kinase 3 Alpha Pathway Related Proteins and Senile Plaque Deposition in Cortex of APP/PS1 Mice

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