《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (03): 136-138.

• 专题研究 • 上一篇    下一篇

脑梗死大鼠康复训练后脑功能恢复及免疫组织化学改变

唐强1; 秦颖2; 倪金霞1; 王德生3   

  1. 1.黑龙江中医药大学附属第二医院康复医学科 黑龙江哈尔滨市 150001;2.哈尔滨医科大学第一临床医学院干部病房 黑龙江哈尔滨市 150001;3.合作导师
  • 收稿日期:2002-12-09 出版日期:2003-03-25 发布日期:2003-03-25

Cerebral functions and immunohistochemical changes caused by rehabilitation in rats after infarction

TANG Qiang,QIN Ying,NI Jin-xia,et al   

  1. Department of Rehabilitation Medicine,the 2nd Hospital of Heilongjiang University of Chinese Traditional Medcine,Harbin 150001,Heilongjiang,China
  • Received:2002-12-09 Published:2003-03-25 Online:2003-03-25

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被引次数

32

摘要： 目的研究康复对急性脑梗死大鼠脑功能恢复的作用机制。方法采用抽签法将30只雄性Wister大鼠随机分为3组:A组(假手术组)、B组(造模组)、C组(康复组);运用血管内栓线法制备脑缺血动物模型;术后24h、3d及7d分别进行Bederson神经功能评分,平衡木、转棒、网屏测评,并于术后第7天测定脑组织内神经生长因子(NGF)、脑源性神经生长因子(BDNF)、转化生长因子(NGF)、碱性成纤维细胞生长因子(bFGF)。结果A组大鼠Bederson神经功能评分无改变,其他两组在术后24h出现评分升高,随时间延长呈逐渐恢复趋势,但各时间点与A组均有高度显著性差异(P<0.01);各组大鼠术后24h均出现抓握、行走及协调能力障碍,组间两两比较,无显著性差异(P>0.05);随着时间的延长,A组的各项功能很快恢复,B、C组也有一定程度的改善,但远不及A组,差异显著(P<0.01);与B组比较,缺血后3d,C组平衡木试验有显著性差异(P<0.05);缺血后7d,除Berderson评分外 ,均有显著性差异。A组与B组术后7d皮层及海马部位均有少量的NGF、BDNF、TGF及bFGF表达,两组间比较无显著性差异(P>0.05),C组NGF、BDNF、TGF及bFGF表达明显升高 (P<0.01)。结论康复能提高脑梗死大鼠平衡、行走及抓握能力,增加患侧肢体的肌力，诱导缺血周边区NGF、BDNF、TGF、bFGF等神经营养因子(NTFs)的表达。

关键词: 脑梗死, 康复, 神经营养因子(NTFs), 大鼠

Abstract: ObjectiveTo study the rehabilitation mechanism of acute cerebral infarction.Methods30 male Wistar rats were randomly divided into 3 groups:group A(sham group),group B(model group) and group C(rehabilitation group).Animal model was made by the middle cerebral artery occlusion(MCAO).For each group,Bederson Neural Function was scored and balancing wood,rotating bar,and net screen were tested24 hours,3 days and 7 days after operation respectively.The nerve growth factor(NGF),brain derived neurotrophic factor(BDNF),transforming growth factor(TGF),basic fibroblast growth factor(bFGF)in brain tissues were tested.ResultsBederson neural function scores of group A had no changes,while those of the other two groups increased after 24 hours and had a tendency to restore gradually with time continuing,but indicated significant differences at each time,compared with group A(P<0.01).The abilities of grasping ,walking and coordinating of all the groups after 24 hours by operation handicapped,and there was no difference among the groups(P>0.05);with time continuing,every function of group A restored soon and that of group B or C improved,to some degree,but showed significant difference to group A(P<0.01).After 3 days by operation,balancing wood test of group C was better than that of group B(P<0.05);after 7 days,except Bederson neural function score,there was difference between the group C and B,that is,the former was superior to the latter. After 7 days by operation,there were a few NGF,BDNF,TGF and bFGF expressions in hippocampus and cortex areas of group A and B,and there was no difference between this two groups(P>0.05),while those of group C greatly increased,compared with group A and B(P<0.05-0.01).ConclusionsRehabilitation therapy can improve the MCAO rats' ability of equilibrating,grasping and walking and strengthen the myodynamia,as well as induce the expression of NGF,BDNF,bFGF and TGF in cortex and hippocampus.

Key words: cerebral infarction, rehabilitation, neurotrophic factors(NTFs), rats