目的 探讨致纤维化细胞因子和炎症细胞因子在冻结肩发生中的可能作用。方法 2014年9月至2016年4月,20例冻结肩患者接受肩关节镜手术,其中原发性冻结肩10例(A组),继发性冻结肩10例(B组)。以同期接受肩关节镜手术的10例非冻结肩患者作为对照(C组)。qPCR检测肩关节滑膜组织中基质金属蛋白酶1(MMP1)、MMP3、肿瘤坏死因子-α (TNF-α)、白细胞介素-1 (IL-1)、IL-6、IL-8、粒细胞巨噬细胞集落刺激因子(GM-CSF)和巨噬细胞集落刺激因子(M-CSF) mRNA表达水平。结果 A组和B组MMP1和MMP3 mRNA表达高于C组(P<0.05),TNF-α、IL-1、IL-6、IL-8、GM-CSF和M-CSF mRNA显著高于C组(P<0.001),A组和B组间各因子mRNA表达无显著性差异(P>0.05)。结论 冻结肩的发生可能与患者关节滑膜组织中致纤维化细胞因子和炎症细胞因子的高表达有关。
Objective To explore the role of fibrogenic cytokines and inflammatory cytokines in the pathogenesis of frozen shoulder. Methods From September, 2014 to April, 2016, 20 patients with frozen shoulder accepted arthroscopic surgery were included, ten of them were diagnosed as primary frozen shoulder (group A), the other ten were secondary frozen shoulder (group B). Other ten patients undergoing shoulder arthroscopy for instability (4 cases), rotator cuff injury (3 cases) and subacromial impingement (3 cases) were as the controls (group C). The mRNA levels of matrix metalloproteinase (MMP) 1, MMP3, tumour necrosis factor-α (TNF-α), interleukin (IL)-1, IL-6, IL-8, granulocyte-macrophage colony stimulating factor (GM-CSF) and M-CSF in synovium were analyzed with quantitative polymerase chain reaction. Results The expression of mRNA of MMP1, MMP3, TNF-α, IL-1, IL-6, IL-8, GM-CSF and M-CSF were more in group A and group B than in group C (P<0.05). There was no significant difference between group A and group B (P>0.05). Conclusion The fibrogenic cytokines and inflammatory cytokines may play a role in the pathogenesis of frozen shoulder.
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