目的观察慢性应激抑郁模型大鼠血浆凝血功能变化以及电针的干预作用。方法雄性Sprague-Dawley大鼠32只,适应性饲养1周后,随机分成空白组、模型组、氟西汀组、电针组,每组8只。后三组采用慢性应激刺激结合孤养21 d建立大鼠抑郁模型,氟西汀组、电针组分别同时采用氟西汀、电针进行干预。测定各组大鼠凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(FIB);酶联免疫吸附测定检测大鼠6酮前列腺素F1α (6-keto-PGF1α)、P-选择素、血栓烷素B2 (TXB2)的变化。结果模型组旷场试验水平运动次数和垂直运动次数较空白组明显降低(P<0.01),电针组和氟西汀组较模型组明显增加(P<0.01)。与空白组比较,模型组PT、APTT、TT明显缩短(P<0.01),FIB含量升高(P<0.05);血清6-keto-PGF1α含量降低(P<0.05),P-选择素、TXB2含量明显升高(P<0.01);与模型组比较,氟西汀组、电针组PT、APTT、TT延长(P<0.05),血清6-keto-PGF1α含量升高(P<0.01), P-选择素、TXB2含量降低(P<0.05),电针组FIB含量降低(P<0.05)。结论慢性应激抑郁模型大鼠凝血功能增强,血小板活化功能异常,电针和氟西汀可改善这些变化。
ObjectiveTo observe the change of coagulation function in chronic stress depression model rats and the effects of electroacupuncture (EA) on it. MethodsA total of 32 Sprague-Dawley rats were randomly divided in control group (n=8), model group (n=8), EA group (n=8) and fluoxetine group (n=8) after a week of acclimation. The 21-day unpredictable mild stress combined with solitarily feeding was used to make the depression model in the latter three groups, meanwhile EA group and the fluoxetine group accepted EA and fluoxetine. They were measured the prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB). Serum levels of 6-keto-prostaglandin F1α (6-keto-PGF1α), P-selectin, thromboxane B2 (TXB2) were determined with enzyme-linked immunoabsorbent assay. ResultsThe cross and rear scores were significantly lower in the model group than in the control group (P<0.01). The scores increased in EA group and the fluoxetine group (P<0.01) compared with the model group. Compared with the control group, PT, APTT and TT decreased (P<0.01), FIB increased (P<0.05), the level of 6-keto-PGF1α decreased, the level of P-selectin and TXB2 increased (P<0.01) in the model group. Compared with the model group, PT, APTT and TT increased (P<0.05), the level of 6-keto-PGF1α increased (P<0.01), and the level of P-selectin and TXB2 decreased (P<0.05) in EA group and the fluoxetine group, and FIB decreased in EA group (P<0.05). ConclusionThe chronic stress depression rats are impaired in the coagulation function and platelet activation, and can be allayed with electroacupuncture and fluoxetine.
[1] 夏青,赵军. 调神解郁针法治疗郁证疗效观察[J]. 上海针灸杂志, 2013, 32(5): 352-353.
[2] 王训强,楼忠泽,张庆玉,等. 血小板功能在抑郁症与心血管疾病间作用的研究进展[J]. 浙江医学, 2016, 38(6): 440-442.
[3] 汤球,刘志学,崔淑芳,等. 大鼠抑郁症模型的建立与评价[J]. 实验动物科学, 2011, 28(1): 6-9.
[4] 薛涛,邬丽莎,刘新民,等. 抑郁症动物模型及评价方法研究进展[J]. 中国实验动物学报, 2015, 23(3): 321-326.
[5] 王建,许崇涛. 抑郁症、焦虑症与心血管疾病和血小板活化功能的关联研究进展[J]. 汕头大学医学院学报, 2013, 26(3): 169-172.
[6] 叶士勇,曾春来. 血小板活化与心血管疾病[J]. 心脑血管病防治, 2012, 12(3): 169-172.
[7] 鲁文波,申光范,李颖,等. 度洛西汀治疗抑郁症后对血小板的影响[J]. 中国药物滥用防治杂志, 2015, 21(6): 336-338.
[8] Ormonde do Carmo MB, Mendes-Ribeiro AC, Matsuura C, et al. Major depression induces oxidative stress and platelet hyperaggregability [J]. J Psychiatr Res, 2015, 61: 19-24.
[9] 杨斌,张玉堂,王莉,等. 抑郁症患者血小板活化功能的临床研究[J]. 中华行为医学与脑科学杂志, 2009, 18(7): 613-614.
[10] Lederbogen F, Gilles M, Maras A, et al. Increased platelet aggregability in major depression? [J]. Psychiatry Res, 2001, 102(3): 255-261.
[11] Serebruany VL, Glassman AH, Malinin AI, et al. Platelet/endothelial biomarkers in depressed patients treated with the selective serotonin reuptake inhibitor sertraline after acute coronary events: the Sertraline AntiDepressant Heart Attack Randomized Trial (SADHART) Platelet Substudy [J]. Circulation, 2003, 108(8): 939-944.
[12] Lopez-Vilchez I, Serra-Millas M, Navarro V, et al. Prothrombotic platelet phenotype in major depression: downregulation by antidepressant treatment [J]. J Affect Disord, 2014, 159: 39-45.
[13] 袁廷伟,孙芳玲,王晓峰,等. 莫诺苷对局灶性脑缺血再灌注大鼠凝血四项的影响[J]. 中国康复理论与实践, 2013, 19(2): 104-106.
[14] 张青萍,刘泰,周军,等. 疏血通脉胶囊对大鼠下腔静脉血栓干湿重及APTT、PT、TT、FIB的影响研究[J]. 时珍国医国药, 2014. 25(4): 824-825.
[15] 朱黎霞,张英丰. 不同配比黄芪丹参对气虚血瘀证模型大鼠血液流变学及TXB2、6-keto-PGF1α影响的研究[J]. 中国医药导报, 2014, 11(22): 13-16.
[16] 刘洁,白鹤,刘喜平,等. 参参康心胶囊对冠心病气虚血瘀证模型小鼠TXB2、6-keto-PGF1α的影响[J]. 中医研究, 2014, 27(1): 62-64.
[17] 张英,崔向丽,杨萍,等. SSRI和SNRI类抗抑郁药的不良反应[J]. 中国药物警戒, 2010, 7(9): 554-556.
[18] 李光灿,肖召安. 文拉法辛致不良反应文献分析[J]. 中国药业, 2014, 23(23): 97-99.
[19] 刘上上,赵红,毕爽丽,等. 针灸治疗抑郁症的作用机制研究进展[J]. 湖北中医药大学学报, 2016, 18(1): 119-122.
[20] 户丽,梁佳,金树英,等. 针刺治疗抑郁症作用机制近5年研究进展[J]. 针刺研究, 2013, 38(3): 253-258.
[21] 钟宝亮,黄悦勤,李会娟. 针灸治疗抑郁症疗效和安全性的系统评价[J]. 中国心理卫生杂志, 2008, 22(9): 641-647.
[22] 郝晓慧,张建斌,刘海蓉,等. 从督脉探讨扶阳理论在针灸临床中的应用[J]. 吉林中医药, 2013, 33(12): 1198-1201.
[23] 景泉凯,赛音朝克图,姚海江,等. 电针对慢性应激抑郁模型大鼠海马磷酸二酯酶4及其亚型表达的影响[J]. 中国康复理论与实践, 2016, 22(9): 1011-1014.
[24] 申斌,于川. 电针百会、印堂配合背俞穴走罐治疗中风后抑郁49例临床观察[J]. 现代中医临床, 2016, 23(4): 36-49.
[25] 纪倩,李志刚,唐银杉,等. 不同电针刺激对慢性应激抑郁模型大鼠行为学及海马谷氨酸转运体的影响[J]. 针刺研究, 2013, 38(3): 202-207,219.
