《中国康复理论与实践》

中国康复理论与实践 >

2018 , Vol. 24 >Issue 2: 153 - 159

DOI: https://doi.org/10.3969/j.issn.1006-9771.2018.02.007

基础研究

隔日限食疗法对脊髓损伤大鼠的保护作用及其机制

  • 何宇 ,
  • 张安仁 ,
  • 孙年怡 ,
  • 王文春 ,
  • 王志强 ,
  • 田丽君 ,
  • 邹文晨 ,
  • 张志强
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  • 1.中国医科大学附属盛京医院康复中心,辽宁沈阳市 110134;
    2.中国人民解放军成都总医院康复医学科,四川成都市 610083
何宇(1987-),男,汉族,辽宁沈阳市人,硕士,康复治疗师,主要研究方向：物理治疗的循证医学研究及应用。

收稿日期: 2017-12-11

  修回日期: 2018-01-29

  网络出版日期: 2018-03-02

基金资助

国家自然科学基金面上项目(No. 81674044)

收起

Protective Effect and Mechanism of Every-other-day Fasting on Rats with Spinal Cord Injury

  • HE Yu ,
  • ZHANG An-ren ,
  • SUN Nian-yi ,
  • WANG Wen-chun ,
  • WANG Zhi-qiang ,
  • TIAN Li-jun ,
  • ZOU Wen-chen ,
  • ZHANG Zhi-qiang
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  • 1. Rehabilitation Center, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110134, China;
    2. Department of Rehabilitation Medicine, Chengdu General Hospital of Chinese People's Liberation Army, Chengdu, Sichuan 610083, China

Received date: 2017-12-11

  Revised date: 2018-01-29

  Online published: 2018-03-02

Supported by

; Supported by National Natural Science Foundation of China (General) (No. 81674044)

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摘要

目的 探讨隔日限食(EODF)对脊髓损伤大鼠脊髓组织病理变化和运动功能恢复的影响及其相关机制。方法 将36只Sprague-Dawley大鼠随机分为假手术组、假手术+EODF组、脊髓损伤组和脊髓损伤+EODF组,每组9只,采用医用动脉瘤夹制备T10脊髓钳夹损伤大鼠模型。于术前1 d及术后1 d、术后第2、4、6、8、10、12周对各组进行BBB评分,术后第12周进行甲苯胺蓝染色。另取180只Sprague-Dawley大鼠,分组同前,每组45只,各组又分为6 h、12 h、1 d、3 d、7 d五个时间点,每个时间点各9只。采用酶联免疫吸附试验检测血清肿瘤坏死因子-α (TNF-α)和白细胞介素-10 (IL-10)水平。结果 脊髓损伤组和脊髓损伤+EODF组BBB评分在术后1 d降至最低(P<0.05),随时间逐渐增加,术后第8、10、12周,脊髓损伤+EODF组优于脊髓损伤组(P<0.05)。术后第12周,脊髓损伤+EODF组较脊髓损伤组脊髓损伤病变程度轻。与假手术组相比,脊髓损伤组术后12 h开始血清TNF-α水平升高(P<0.05),呈先升后降趋势,7 d恢复;术后各时间点血清IL-10水平均升高(P<0.05)。术后1 d,脊髓损伤+EODF组血清TNF-α表达水平低于脊髓损伤组(P<0.05);术后各时间点,脊髓损伤+EODF组与脊髓损伤组间血清IL-10水平无显著性差异(P>0.05)。结论 长期EODF可促进脊髓损伤大鼠后肢运动功能障碍的恢复,减轻脊髓损伤后的病理损害程度,对脊髓损伤急性期炎性反应有一定的抑制作用,可能是其发挥神经保护作用的基础。

关键词： 脊髓损伤; 隔日限食; 运动功能; 组织结构; 炎性反应; 大鼠

本文引用格式

何宇 , 张安仁 , 孙年怡 , 王文春 , 王志强 , 田丽君 , 邹文晨 , 张志强 . 隔日限食疗法对脊髓损伤大鼠的保护作用及其机制[J]. 中国康复理论与实践, 2018 , 24(2) : 153 -159 . DOI: 10.3969/j.issn.1006-9771.2018.02.007

Abstract

Objective To observe the effect of every-other-day fasting (EODF) on pathology and functional recovery of rats with spinal cord clip-compression injury, and to explore the related mechanism.Methods A total of 36 Sprague-Dawley rats were randomly assigned to groups A (sham operation), B (sham operation and EODF), C (spinal cord injury) and D (spinal cord injury and EODF) with nine rats in each group. The spinal cord injury rat model in T10 was established by using medical aneurysm clip in latter two groups. The motor function was assessed by Basso-Beattie-Bresnahan (BBB) score one day before and one day, two, four, six, eight, ten, twelve weeks after operation; and toluidine blue staining was performed for pathological observation at twelve weeks after operation. Another 180 Sprague-Dawley rats were randomly assigned in same way. The level of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were detected with ELISA six hours, twelve hours, one day, three days and seven days after operation.Results The BBB score reached lowest on the first day in groups C and D (P<0.05), and gradually increased with time, and were higher in group D than in group C eight weeks, ten weeks and twelve weeks after operation (P<0.05). The pathology in spinal cord was less in group D than in group C. Compared with group A, the level of serum TNF-α increased twelve hours after operation (P<0.05), peaked one day after operation, and returned back seven days after operation; the level of serum IL-10 increased at each time point after operation (P<0.05). Compared with group C, the level of serum TNF-α was lower in group D one day after operation (P<0.05); the level of serum IL-10 was not significantly different at each time point after operation (P>0.05).Conclusion Long-term EODF can promote the hind limb motor recovery in rats with spinal cord clip-compression injury, and alleviate pathological damage. EODF might inhibit acute inflammatory reaction at acute stage of spinal cord injury, which may be correlated with its neuroprotective effect.

Key words： spinal cord injury; every-other-day fasting; motor function; tissue structure; inflammatory response; rats

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