目的 利用生物信息学方法对男女肌肉衰减综合征患者与正常人群进行差异基因分析,研究肌肉衰减个体骨骼肌细胞基因表达在性别间的差异。方法 从Gene Expression Omnibus数据库下载老年肌肉衰减综合征相关数据集,采用BRB-Array Tools及STRING软件在线分析,利用Cytoscape软件进行蛋白质相互作用网络分析。结果 男性差异表达基因219个,其中高表达152个,低表达67个;女性差异表达基因142个,其中高表达90个,低表达52个;共同高表达47个,共同低表达21个。基因本体(GO)分析显示,女性肌肉衰减综合征患者差异基因涉及的GO类别较多,共同的富集模块为脂肪细胞的分化调节、蛋白激酶抑制剂活性、蛋白激酶调节剂活性等。抗肌萎缩蛋白、波形蛋白和原肌球蛋白α-3链是男性蛋白质相互作用网络中重要节点,而金属硫蛋白1H、动力蛋白轻链为女性的重要节点。结论 老年肌肉衰减综合征发生机制存在性别差异。针对各自重要节点的后续研究可能对老年肌肉衰减综合征的发生机制及并发症的研究起重要作用。
Objective To explore the gender differences in the genes expression of sarcopenia with bioinformatics. Methods The gene expression profiles downloaded from Gene Expression Omnibus database were analysed with BRB-Array Tools and STRING, then the protein-protein interaction network was built with Cytoscape. Results There were 152 genes up-regulated and 67 genes down-regulated in sarcopenic men, and 90 up-regulated and 52 down-regulated in women, with the same 47 up-regulated and 21 down-regulated. The gene ontology (GO) terms were found to be more complex in the sarcopenic women. The function analysis showed the same module genes were enriched in regulation of fat cell differentiation, protein kinase inhibitor activity and protein kinase regulator activity. In the protein-protein interaction networks, dystrophin, vimentin and tropomyosin α-3 were the most important in men, and metallothionein 1H and dynein light chain in women.Conclusion The nosogenesis of sarcopenia is different between genders from differentially expressed genes, that may be important for the future study.
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