目的 探讨电针延缓大鼠失神经骨骼肌萎缩的可能机制。方法 21只雄性Sprague-Dawley大鼠随机分为假手术组(n=7)、模型组(n=7)和电针组(n=7)。后两组切断右侧坐骨神经制备大鼠失神经骨骼肌萎缩模型。造模后1 d,电针组电针术侧足三里和环跳穴。干预8周后取双侧腓肠肌,称重,计算各组大鼠腓肠肌湿重比;HE染色测量肌纤维截面积和直径;逆转录实时定量聚合酶链反应检测大鼠术侧腓肠肌中自噬相关基因ULK1、Atg13、Beclin1、Atg14、Atg7、Atg12、Atg5和Atg16L1 mRNA表达。结果 与假手术组相比,模型组和电针组术侧腓肠肌湿重比、肌纤维截面积及直径显著下降(P<0.001),但电针组高于模型组(P<0.05)。与假手术组相比,模型组术侧腓肠肌中ULK1、Atg13、Beclin1、Atg14、Atg7、Atg12、Atg5和Atg16L1 mRNA表达量显著升高(P<0.001);与模型组相比,电针组以上mRNA表达量均下降(P<0.05)。结论 电针干预可能通过调控自噬相关基因的表达,抑制自噬过度激活,从而维持骨骼肌细胞稳态,延缓失神经骨骼肌萎缩。
Objective To explore the effects and mechanism of electroacupuncture (EA) on denervation-induced atrophy. Methods A total of 21 male Sprague-Dawley rats were divided into sham group (n=7), model group (n=7) and EA group (n=7). The latter two groups were cut off their right sciatic nerve. Since one day after modeling, EA group accepted electroacupuncture at right Zusanli (ST36) and Huantiao (GB30) for eight weeks. Then, the gastrocnemius of all the rats were obtained, and measured the wet mass ratio. Cross-sectional area (CSA) and fiber diameter were measured after HE staining. The expression of autophagy-related gene ULK1, Atg13, Beclin1, Atg14, Atg7, Atg12, Atg5 and Atg16L1 were tested with reverse transcription real-time quantitative polymerase chain reaction. Results Compared with the sham group, the wet mass ratio, CSA and fiber diameter of gastrocnemius were lower significantly in the model group and EA group (P<0.001), and they were more in EA group than in the model group (P<0.05). Compared with the sham group, the mRNA expression of ULK1, Atg13, Beclin1, Atg14, Atg7, Atg12, Atg5 and Atg16L1 was more significantly in the model group (P<0.001), and they decreased in EA group compared with those of the model group (P<0.05).Conclusion Electroacupuncture can inhibit the overexpression of autophagy-related gene in denervated rats, which may steady skeletal muscle cells to delay atrophy.
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